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首页> 外文期刊>Cell and Tissue Research >Completion of meiosis in male zebrafish (Danio rerio) despite lack of DNA mismatch repair gene mlh1
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Completion of meiosis in male zebrafish (Danio rerio) despite lack of DNA mismatch repair gene mlh1

机译：尽管缺少DNA错配修复基因mlh1，雄性斑马鱼（Danio rerio）的减数分裂完成

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摘要

Mlh1 is a member of DNA mismatch repair (MMR) machinery and is also essential for the stabilization of crossovers during the first meiotic division. Recently, we have shown that zebrafish mlh1 mutant males are completely infertile because of a block in metaphase I, whereas females are fertile but have aneuploid progeny. When studying fertility in males in a two-fold more inbred background, we have however observed low numbers of fertilized eggs (approximately 0.4%). Histological examination of the testis has revealed that all spermatogenic stages prior to spermatids (spermatogonia, primary spermatocytes, and secondary spermatocytes) are significantly increased in the mutant, whereas the total weight of spermatids and spermatozoa is highly decreased (1.8 mg in wild-type vs. 0.1 mg in mutants), a result clearly different from our previous study in which outbred males lack secondary spermatocytes or postmeiotic cells. Thus, a delay of both meiotic divisions occurs rather than complete arrest during meiosis I in these males. Eggs fertilized with mutant sperm develop as malformed embryos and are aneuploid making this male phenotype much more similar to that previously described in the mutant females. Therefore, crossovers are still essential for proper meiosis, but meiotic cell divisions can progress without it, suggesting that this mutant is a suitable model for studying the cellular mechanisms of completing meiosis without crossover stabilization.
机译：Mlh1是DNA错配修复（MMR）机制的成员，也是在第一次减数分裂分裂过程中稳定交换的关键。最近，我们已经显示，由于中期I的阻滞，斑马鱼mlh1突变体雄性完全不育，而雌性则是可育的，但具有非整倍体后代。当研究近交背景两倍的雄性育性时，我们观察到受精卵的数量很少（约0.4％）。睾丸的组织学检查显示，突变体中精子之前的所有生精阶段（精原细胞，初级精细胞和次级精细胞）均显着增加，而精子和精子的总重量却大大降低（野生型vs精子为1.8 mg突变体中加入0.1 mg），这一结果与我们以前的研究明显不同，在以前的研究中，远交的男性缺少继发的精母细胞或减数分裂后的细胞。因此，在这些雄性的减数分裂I期间，两种减数分裂分裂均发生延迟而不是完全停止。用突变精子受精的卵会发育为畸形的胚胎，并且是非整倍体，使得这种雄性表型与先前在突变雌性中描述的更为相似。因此，交叉对于适当的减数分裂仍然是必不可少的，但是减数分裂的细胞分裂可以继续进行，这表明该突变体是研究没有减数分裂稳定的完成减数分裂的细胞机制的合适模型。

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  • 来源
    《Cell and Tissue Research》 |2008年第1期|133-139|共7页
  • 作者

    Marcelo C. Leal; Harma Feitsma; Edwin Cuppen; Luiz R. Fran?a; Rüdiger W. Schulz;

  • 作者单位

    Science Faculty Department Biology Section Endocrinology ampamp Metabolism Utrecht University Kruyt Building Room Z-203 Padualaan 8 NL-3584 CH Utrecht The Netherlands;

    Hubrecht Institute for Developmental Biology and Stem Cell Research Section Functional Genomics and Bioinformatics Uppsalalaan 8 3584 CT Utrecht The Netherlands;

    Hubrecht Institute for Developmental Biology and Stem Cell Research Section Functional Genomics and Bioinformatics Uppsalalaan 8 3584 CT Utrecht The Netherlands;

    Laboratory of Cellular Biology Department of Morphology Federal University of Minas Gerais Minas Gerais Brazil;

    Science Faculty Department Biology Section Endocrinology ampamp Metabolism Utrecht University Kruyt Building Room Z-203 Padualaan 8 NL-3584 CH Utrecht The Netherlands;

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  • 正文语种 eng
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  • 关键词

    DNA repair enzyme Mlh1; Testis; Spermatogenesis; Male fertility; Aneuploidy; Zebrafish, Danio rerio (Teleostei);

    机译：DNA修复酶Mlh1;睾丸;精子发生;雄性育性;非整倍性;斑马鱼;达尼奥（Teleostei）;

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