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首页> 外文期刊>Cell and Tissue Research >Endothelial cell suppression of peripheral blood mononuclear cell trafficking in vitro during acute exposure to feline immunodeficiency virus
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Endothelial cell suppression of peripheral blood mononuclear cell trafficking in vitro during acute exposure to feline immunodeficiency virus

机译：急性暴露于猫免疫缺陷病毒期间体外抑制外周血单个核细胞运输的内皮细胞

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摘要

Trafficking of peripheral blood mononuclear cells (PBMCs) into the brain is a critical step in the initiation of human immunodeficiency virus (HIV)-associated central nervous system disease. To examine potential factors that control trafficking during the earliest stages of infection, PBMC transmigration across a cultured feline brain endothelial cell (BECs) monolayer was measured after selective exposure of various cell types to feline immunodeficiency virus (FIV). Infection of the PBMCs with FIV increased the trafficking of monocytes and CD4 and CD8 T cells. Additional exposure of the BECs to FIV suppressed mean monocyte, CD4 T cell, and CD8 T cell trafficking. B cell trafficking was unaltered by these changing conditions. Subsequent exposure of astrocytes or microglia to FIV altered transmigration of different PBMC subsets in different ways. Treated microglia compared with treated astrocytes decreased monocyte transmigration, whereas B cell transmigration was increased significantly. When both astrocytes and microglia were exposed to FIV, an increase in CD8 T cell transmigration relative to BECs alone, to BECs plus astrocytes, or to BECs plus microglia was demonstrated. Thus, initial exposure of PBMCs to FIV is sufficient to induce a general increase in trafficking, whereas initial exposure of endothelial cells to FIV tends to down-regulate this effect. Selectivity of trafficking of specific PBMC subsets is apparent only after exposure of cells of the central nervous system to FIV in co-culture with the endothelium.
机译：在人类免疫缺陷病毒（HIV）相关的中枢神经系统疾病的起始过程中，将外周血单核细胞（PBMC）贩运到大脑是至关重要的一步。为了检查在感染的早期阶段控制贩运的潜在因素，在将各种细胞类型选择性暴露于猫免疫缺陷病毒（FIV）之后，测量了培养的猫脑内皮细胞（BEC）单层的PBMC迁移。用FIV感染PBMC会增加单核细胞以及CD4和CD8 T细胞的运输。 BEC进一步暴露于FIV会抑制平均单核细胞，CD4 T细胞和CD8 T细胞的运输。这些变化的条件不会改变B细胞的运输。星形胶质细胞或小胶质细胞随后暴露于FIV以不同的方式改变了不同PBMC亚群的转运。与处理过的星形胶质细胞相比，处理过的小胶质细胞减少了单核细胞的迁移，而B细胞的迁移显着增加。当星形胶质细胞和小胶质细胞都暴露于FIV时，相对于单独的BEC，BEC加星形胶质细胞或BECs和小胶质细胞，CD8 T细胞的迁移增加。因此，PBMC最初暴露于FIV足以引起运输的普遍增加，而内皮细胞最初暴露于FIV则倾向于下调这种作用。仅在与内皮共培养中枢神经系统细胞暴露于FIV后，特定PBMC子集的运输选择性才显而易见。

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  • 来源
    《Cell and Tissue Research》 |2008年第1期|55-65|共11页
  • 作者

    Lola C. Hudson; Mary B. Tompkins; Rick B. Meeker;

  • 作者单位

    Department of Molecular Biomedical Sciences College of Veterinary Medicine 4700 Hillsborough Street Raleigh NC 27606 USA;

    Immunology Program North Carolina State University Raleigh NC 27606 USA;

    Department of Neurology School of Medicine University of North Carolina Chapel Hill NC 27599 USA;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    HIV; Astrocytes; Microglia; Monocyte; T cell; Feline;

    机译：HIV;星形胶质细胞;小胶质细胞;单核细胞;T细胞;猫;

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