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首页> 外文期刊>Cell and Tissue Research >Injections of leptin into rat ventromedial hypothalamus increase adipocyte apoptosis in peripheral fat and in bone marrow
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Injections of leptin into rat ventromedial hypothalamus increase adipocyte apoptosis in peripheral fat and in bone marrow

机译：向大鼠腹膜下丘脑注射瘦素可增加周围脂肪和骨髓中脂肪细胞的凋亡

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The accumulation of fat cells (adipocytes) in bone marrow is now thought to be a factor contributing to age-related bone loss. Women with osteoporosis have higher numbers of marrow adipocytes than women with healthy bone, and bone formation rate is inversely correlated with adipocyte number in bone tissue biopsies from both men and women. Adipogenic differentiation of bone marrow stromal cells increases with age, but the factors regulating populations of mature adipocytes are not well understood. Leptin is thought to regulate adipose tissue mass via its receptors in the ventromedial hypothalamus (VMH). We have therefore tested the hypothesis that stimulation of leptin receptors in the VMH regulates adipocyte number in bone marrow. Results indicate that unilateral twice-daily injections of leptin into the rat VMH for only 4 or 5 days cause a significant reduction in the number of adipocytes in peripheral fat pads and bone marrow and indeed eliminate adipocytes almost entirely from bone marrow of the proximal tibia. Osteoblast surface is not affected with leptin treatment. Apoptosis assays performed on bone marrow samples from control and treated rats have revealed a significant increase in protein concentration of the apoptosis marker caspase-3 with leptin treatment. We conclude that stimulation of leptin receptors in the VMH significantly decreases the adipocyte population in bone marrow, primarily through apoptosis of marrow adipocytes. Elimination of marrow adipocytes via this central pathway may represent a useful strategy for the treatment and prevention of osteoporosis.
机译：现在认为，脂肪细胞（脂肪细胞）在骨髓中的积累是导致与年龄有关的骨质流失的因素。患有骨质疏松症的女性比具有健康骨骼的女性拥有更多的骨髓脂肪细胞，并且在男性和女性的骨组织活检中，骨形成率与脂肪细胞数量成反比。骨髓基质细胞的成脂分化随年龄增长而增加，但调节成熟脂肪细胞群体的因素尚不十分清楚。瘦素被认为通过腹侧下丘脑（VMH）中的受体调节脂肪组织质量。因此，我们测试了以下假设：VMH中的瘦素受体刺激调节骨髓中的脂肪细胞数量。结果表明，单次每天两次向大鼠VMH中注射瘦素持续4或5天，即可导致外周脂肪垫和骨髓中脂肪细胞的数量显着减少，实际上几乎完全消除了胫骨近端骨髓中的脂肪细胞。瘦素处理不影响成骨细胞表面。对来自对照和治疗的大鼠的骨髓样品进行的细胞凋亡分析表明，用瘦素处理后，凋亡标记caspase-3的蛋白质浓度显着增加。我们得出的结论是，刺激VMH中的瘦素受体可显着减少骨髓中的脂肪细胞，主要是通过骨髓脂肪细胞的凋亡。通过该中央途径消除骨髓脂肪细胞可能代表治疗和预防骨质疏松症的有用策略。

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