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首页> 外文期刊>Cell and Tissue Research >A comprehensive study of the spatial and temporal expression of the col5a1 gene in mouse embryos: a clue for understanding collagen V function in developing connective tissues
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A comprehensive study of the spatial and temporal expression of the col5a1 gene in mouse embryos: a clue for understanding collagen V function in developing connective tissues

机译：col5a1基因在小鼠胚胎中的时空表达的全面研究：了解胶原V在结缔组织发育中的功能的线索

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摘要

Collagen V is a quantitatively minor component of collagen I fibrils and the defective product of classic Ehlers-Danlos syndrome (EDS). To provide new insights into its embryonic function, a continuous evaluation of the expression pattern of proα1(V), a chain common to all collagen V molecular forms, was performed by in situ hybridization of developing mouse from 7.5 days after conception (dpc) to birth. Proα1(V) transcripts were first detected at 8.5 dpc, signals being considerably augmented at 16.5 dpc and declining at birth. Hybridization signals were, at first, exclusively detected in the dorsal aorta wall, heart, and adnexa. At 10.5 dpc, col5a1 expression was found in the heart, dorsal aorta wall, branchial arches, mesonephrotic tubules, and intestinal mesenchyme and coincided with proα1(I) developmental expression. Later stages exhibited an intense signal in more restricted regions, notably the skin, the bones and vertebral column, the cornea, the tendons and ligaments, the peritoneal membranes, the umbilical cord, and the salivary gland. The data revealed the important contribution of collagen V to the development of functional connective tissues. Proα1(V) signals were exclusively detected in the flattened cells of the surface ectoderm at 10.5 dpc. By 12.5 dpc, when cells had become cuboidal, the signal switched to the dermal fibroblasts. Thus, type V collagen appears to contribute to epidermis differentiation. Our data also suggest that collagen V participates in bone formation and/or mineralization and in the renewal of stromal cells in the cornea. The results underscore the role of collagen V in developing embryos and provide important clues for analyzing the phenotype of mouse models for EDS.
机译：V型胶原蛋白是I型胶原蛋白原纤维中的微量成分，也是经典的Ehlers-Danlos综合征（EDS）的缺陷产物。为了提供对其胚胎功能的新见解，通过对发育中的小鼠从受孕后（dpc）到7.5天进行原位杂交，对proα1（V）（所有胶原V分子形式共有的一条链）的表达模式进行了连续评估。出生。 Proα1（V）转录本最初在8.5 dpc时被检测到，信号在16.5 dpc时显着增加，在出生时下降。首先，仅在背主动脉壁，心脏和附件中检测到杂交信号。在10.5 dpc时，在心脏，背主动脉壁，branch弓，中肾小管和肠间充质中发现了col5a1表达，并且与proα1（I）的发育表达相吻合。后期在更受限的区域表现出强烈的信号，特别是皮肤，骨头和椎骨，角膜，肌腱和韧带，腹膜，脐带和唾液腺。数据显示胶原蛋白V对功能性结缔组织的发育的重要贡献。仅在表面外胚层的扁平细胞中以10.5 dpc的水平检测到Proα1（V）信号。到12.5 dpc时，当细胞变成长方体时，信号切换到真皮成纤维细胞。因此，V型胶原似乎有助于表皮分化。我们的数据还表明，胶原蛋白V参与了角膜的骨形成和/或矿化以及基质细胞的更新。这些结果强调了胶原蛋白V在胚胎发育中的作用，并为分析EDS小鼠模型的表型提供了重要线索。

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  • 来源
    《Cell and Tissue Research》 |2007年第2期|323-332|共10页
  • 作者

    Muriel Roulet; Florence Ruggiero; Gérard Karsenty; Dominique LeGuellec;

  • 作者单位

    Institut de Biologie et Chimie des Protéines CNRS UMR 5086 IFR 128 BioSciences Lyon-Gerland Université Lyon 1 7 passage du Vercors 69367 Lyon Cedex 7 France;

    Institut de Biologie et Chimie des Protéines CNRS UMR 5086 IFR 128 BioSciences Lyon-Gerland Université Lyon 1 7 passage du Vercors 69367 Lyon Cedex 7 France;

    Department of Molecular and Human Genetics Bone Disease Program of Texas Houston 77030 USA;

    Institut de Biologie et Chimie des Protéines CNRS UMR 5086 IFR 128 BioSciences Lyon-Gerland Université Lyon 1 7 passage du Vercors 69367 Lyon Cedex 7 France;

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  • 正文语种 eng
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  • 关键词

    Type V collagen; Extracellular matrix; Development; In situ hybridization; Mouse;

    机译：V型胶原;细胞外基质;发育;原位杂交;小鼠;

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