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首页> 外文期刊>Cell and Tissue Research >Nucleus accumbens subregions: hodological and immunohistochemical study in the domestic chick (Gallus domesticus)
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Nucleus accumbens subregions: hodological and immunohistochemical study in the domestic chick (Gallus domesticus)

机译:伏伏核子区域:家禽(Gallus domesticus)的流变学和免疫组化研究

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The nucleus accumbens was identified in avian species some time ago. However, the precise localization and extent of this nucleus is still a matter of controversy. We have used immunolabeling against calbindin, neuropeptide Y, and DARPP-32 (dopamine- and adenosine-related phosphoprotein, 32 kDa) for the selective marking of putative accumbens subdivisions and have followed the anterograde transport of biotinylated dextran amine injected to the nucleus tractus solitarii region of 7-day-old domestic chicks. The nucleus accumbens extending between rostrocaudal atlas coordinates A 10.6 and A 8.8 can be subdivided into the core and shell, the core corresponding to the ventromedial and juxtaventricular medial striatum laterodorsal to the bed nucleus of stria terminalis, and the shell representing an arched region situated ventrally and ventrolaterally to the core. Immunoreactivity to both calbindin and neuropeptide Y is more intense in the shell than in the core division. DARPP-32 immunolabeling does not differ in the two divisions but is markedly weaker in the bed nucleus of stria terminalis, enabling the separation of this nucleus from the surrounding accumbens subdivisions. Fibers from the nucleus solitarius predominantly terminate in the shell division, similar to the situation described in mammals. Whereas the suggested core lies entirely within the boundary of the medial striatum, the shell seems partially to overlap the ventral pallidum. We have been unable to subdivide the remaining part of accumbens lying rostral to A 10.6 into a putative shell and core by the methods employed in the present study. This region probably corresponds to the rostral pole of the nucleus accumbens.
机译:前一段时间在鸟类中发现伏伏核。但是,该核的精确定位和范围仍是一个有争议的问题。我们已经使用针对钙结合蛋白,神经肽Y和DARPP-32(多巴胺和腺苷相关的磷酸化蛋白,32 kDa)的免疫标记来选择性地标记伏安区的选择性标记,并遵循生物素化右旋糖酐胺的顺行转运至孤核细胞核7日龄家禽的区域。伏隔核在轮状尾骨图谱坐标A 10.6和A 8.8之间延伸,可细分为核和壳,核对应于背侧纹状体床核的背侧和后室内侧纹状体,而壳代表位于腹侧的弓形区和腹侧到核心。对钙结合蛋白和神经肽Y的免疫反应性在壳中比在核心区中更强。 DARPP-32免疫标记在这两个部分中没有区别,但是在纹状体终末床核中明显较弱,从而使该核与周围伏隔细分区分开。类似于哺乳动物中描述的情况,来自孤核的纤维主要终止于壳分裂。尽管建议的核心完全位于内侧纹状体的边界内,但外壳似乎部分与腹侧苍白球重叠。我们尚无法通过本研究中采用的方法将位于方尖碑的剩余部分降到A 10.6分为假定的壳和核。该区域可能对应于伏隔核的延髓极。

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