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首页> 外文期刊>Cell and Tissue Research >Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation
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Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation

机译：通过四倍体胚胎聚集判断，传代数影响小鼠胚胎干细胞的多能性

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摘要

The aim of this study was to determine whether the number of passages affected the developmental pluripotency of embryonic stem (ES) cells as measured by the attainment of adult fertile mice derived from embryonic stem (ES) cell/tetraploid embryo complementation. Thirty-six newborns were produced by the aggregation of tetraploid embryos and hybrid ES cells after various numbers of passages. These newborns were entirely derived from ES cells as judged by microsatellite DNA, coat-color phenotype, and germline transmission. Although 15 survived to adulthood, 17 died of respiratory failure, and four were eaten by their foster mother. From the 15 mice that reached adulthood and that could reproduce, none arose from ES cells at passage level 15 or more. All 15 arose from cells at passages 3–11. Our results demonstrate that the number of passages affects the developmental pluripotency of ES cells.
机译：这项研究的目的是确定传代数是否影响胚胎干（ES）细胞的发育多能性，方法是通过获得源自胚胎干（ES）细胞/四倍体胚胎互补的成年可育小鼠来衡量。经过四倍的传代，四倍体胚胎和杂种ES细胞的聚集产生了36个新生儿。根据微卫星DNA，毛色表型和种系传播判断，这些新生儿完全来源于ES细胞。尽管有15名幸存者到成年，但有17人死于呼吸衰竭，其中有4人被养母吃掉。在成年并可以繁殖的15只小鼠中，传代水平为15或更高的ES细胞中没有一只出现。所有15个细胞都来自3-11代的细胞。我们的结果表明，传代的次数会影响ES细胞的发育多能性。

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  • 来源
    《Cell and Tissue Research》 |2007年第3期|607-614|共8页
  • 作者

    Xiang-yun Li; Qing Jia; Ke-qian Di; Shu-min Gao; Xiao-hui Wen; Rong-yan Zhou; Wei Wei; Li-ze Wang;

  • 作者单位

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

    College of Life Sciences Peking University Beijing 100871 People’s Republic of China;

    College of Animal Science and Technology Agricultural University of Hebei Baoding Hebei 071001 People’s Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Embryonic stem cells; Tetraploid embryo; Aggregation; Pluripotency; Mouse;

    机译：胚胎干细胞;四倍体胚胎;聚集;多能性;小鼠;

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