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Expression of HMGB1 during tooth development

机译：HMGB1在牙齿发育过程中的表达

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摘要

High mobility group box 1 (HMGB1) is a nuclear and cytosolic protein that can act as a transcription factor, a growth factor, or a cytokine. To elucidate a possible role for HMGB1 in tooth development, we have studied the expression of HMGB1 and its receptor RAGE (receptor for advanced glycation end-products) during the late fetal and early postnatal period of rat by using light- and electron-microscopic immunohistochemistry. Low HMGB1 protein expression was observed during fetal and newborn stages of tooth development. However, from postnatal day 5 (P5) onward, a marked increase occurred in the levels of the protein in most dental cell types. Expression was particularly high in ameloblasts and odontoblasts at regions of ongoing mineralization. Although most HMGB1 immunoreactivity was confined to cell nuclei, it was also present in odontoblast cytoplasm. At P5, ameloblasts and odontoblasts also showed RAGE immunoreactivity, and reverse transcription-polymerase chain reaction demonstrated both HMGB1 and RAGE mRNA in human dental pulp cells in vitro. Immunoblots performed on extracts from bovine dentin demonstrated a principal band at ～27 kDa, indicating that HMGB1 participates in tooth mineralization. The expression of both ligand and receptor suggests an autocrine/paracrine HMGB1 signalling axis in odontoblasts.
机译：高迁移率族盒1（HMGB1）是一种核和胞质蛋白，可以充当转录因子，生长因子或细胞因子。为了阐明HMGB1在牙齿发育中的可能作用，我们使用光镜和电子显微镜免疫组织化学研究了HMGB1及其受体RAGE（在大鼠胎儿晚期和出生后早期）的表达。 。在胎儿和新生牙齿发育阶段，HMGB1蛋白表达较低。但是，从出生后第5天（P5）开始，大多数牙齿细胞类型中的蛋白质水平显着增加。在持续矿化区域的成釉细胞和成牙本质细胞中的表达特别高。尽管大多数HMGB1免疫反应性仅限于细胞核，但它也存在于成牙本质细胞质中。在P5时，成釉细胞和成牙本质细胞也显示RAGE免疫反应性，逆转录聚合酶链反应在体外显示人牙髓细胞中的HMGB1和RAGE mRNA。对牛牙本质提取物进行的免疫印迹显示在〜27 kDa处有一个主要谱带，表明HMGB1参与了牙齿矿化。配体和受体的表达暗示成牙本质细胞中的自分泌/旁分泌HMGB1信号轴。

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  • 来源
    《Cell and Tissue Research》 |2007年第3期|511-519|共9页
  • 作者

    R. Sugars; E. Karlstr?m; C. Christersson; M.-L. Olsson; M. Wendel; K. Fried;

  • 作者单位

    Center for Oral Biology Novum Karolinska Institutet P.O. Box 4064 SE-141 04 Huddinge Sweden;

    Center for Oral Biology Novum Karolinska Institutet P.O. Box 4064 SE-141 04 Huddinge Sweden;

    Center for Oral Biology Novum Karolinska Institutet P.O. Box 4064 SE-141 04 Huddinge Sweden;

    Center for Oral Biology Novum Karolinska Institutet P.O. Box 4064 SE-141 04 Huddinge Sweden;

    Center for Oral Biology Novum Karolinska Institutet P.O. Box 4064 SE-141 04 Huddinge Sweden;

    Center for Oral Biology Novum Karolinska Institutet P.O. Box 4064 SE-141 04 Huddinge Sweden;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    HMGB1; RAGE; Tooth; Development; Odontoblast; Rat (Sprague Dawley); Bovine;

    机译：HMGB1;RAGE;牙齿;发育;成牙本质细胞;Rat（Sprague Dawley）;牛;

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