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首页> 外文期刊>Cell and Tissue Research >Expression of retinaldehyde dehydrogenase (RALDH)2 and RALDH3 but not RALDH1 in the developing anterior pituitary glands of rats
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Expression of retinaldehyde dehydrogenase (RALDH)2 and RALDH3 but not RALDH1 in the developing anterior pituitary glands of rats

机译：在发展中的大鼠垂体前叶中，视黄醛脱氢酶（RALDH）2和RALDH3的表达而不是RALDH1的表达

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摘要

Retinoic acid (RA) plays an important role in cell growth and tissue development and is also a regulating factor of pituitary function. However, whether RA is generated in the pituitary gland and plays a role as a paracrine and/or autocrine hormone is generally unknown. RA is synthesized from retinoids through oxidation processes. Dehydrogenases catalyzing the oxidation of retinal to RA are members of the retinaldehyde dehydrogenase (RALDH) family. In this study, we examined the expression of RALDH1, RALDH2, and RALDH3 mRNA in the rat embryonic pituitary gland. By in situ hybridization with digoxigenin-labeled cRNA probes, we detected mRNA expression for RALDH2 and RALDH3, but not RALDH1. The expression of RALDH2 and RALDH3 was located in Rathke’s pouch at embryonic day 12.5 (E12.5) and subsequently in the developing anterior pituitary gland. We also used quantitative real-time polymerase chain reaction to analyze RALDH2 and RALDH3 mRNA expression levels during the development of the pituitary gland. We found that pituitary RALDH2 and RALDH3 mRNA levels were high at E17.5 and decreased markedly after birth. Our study is the first to show that RALDH2 and RALDH3, but not RALDH1, are expressed in the embryonic anterior pituitary gland of the rat.
机译：维甲酸（RA）在细胞生长和组织发育中起重要作用，并且也是垂体功能的调节因子。然而，RA是否在垂体中产生并起旁分泌和/或自分泌激素的作用通常是未知的。 RA是由类维生素A通过氧化过程合成的。催化视网膜氧化为RA的脱氢酶是视黄醛脱氢酶（RALDH）家族的成员。在这项研究中，我们检查了大鼠胚胎垂体中RALDH1，RALDH2和RALDH3 mRNA的表达。通过与洋地黄毒苷标记的cRNA探针原位杂交，我们检测到RALDH2和RALDH3的mRNA表达，但未检测到RALDH1的mRNA表达。 RALDH2和RALDH3的表达在胚胎第12.5天（E12.5）的Rathke囊中，随后在发育中的垂体前叶腺中表达。我们还使用定量实时聚合酶链反应来分析垂体发育过程中的RALDH2和RALDH3 mRNA表达水平。我们发现垂体RALDH2和RALDH3 mRNA水平在E17.5较高，并且在出生后明显降低。我们的研究首次表明RALDH2和RALDH3，而不是RALDH1在大鼠的胚胎垂体前叶腺中表达。

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  • 来源
    《Cell and Tissue Research》 |2007年第1期|129-135|共7页
  • 作者

    Ken Fujiwara; Fumihiko Maekawa; Motoshi Kikuchi; Shu Takigami; Toshihiko Yada; Takashi Yashiro;

  • 作者单位

    Division of Histology and Cell Biology Department of Anatomy Jichi Medical University School of Medicine 3311-1 Yakushiji Shimotsuke-shi Tochigi 329-0498 Japan;

    Division of Integrative Physiology Department of Physiology Jichi Medical University School of Medicine Tochigi Japan;

    Division of Histology and Cell Biology Department of Anatomy Jichi Medical University School of Medicine 3311-1 Yakushiji Shimotsuke-shi Tochigi 329-0498 Japan;

    Division of Histology and Cell Biology Department of Anatomy Jichi Medical University School of Medicine 3311-1 Yakushiji Shimotsuke-shi Tochigi 329-0498 Japan;

    Division of Integrative Physiology Department of Physiology Jichi Medical University School of Medicine Tochigi Japan;

    Division of Histology and Cell Biology Department of Anatomy Jichi Medical University School of Medicine 3311-1 Yakushiji Shimotsuke-shi Tochigi 329-0498 Japan;

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  • 正文语种 eng
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  • 关键词

    Aanterior pituitary gland; Retinoic acid; RALDH; Development; In situ hybridization; Rat (Wistar);

    机译：垂体前叶;维甲酸;ALDH;发育;原位杂交;大鼠（Wistar）;

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