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首页> 外文期刊>Cell and Tissue Research >Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells
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Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells

机译：大鼠唾液腺腺泡细胞原代培养中claudin合成的差异与细胞的特定三维结构有关

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摘要

Tight junctions are essential for the maintenance of epithelial cell polarity. We have previously established a system for the primary culture of salivary parotid acinar cells that retain their ability to generate new secretory granules and to secrete proteins in a signal-dependent manner. Because cell polarity and cell-cell adhesion are prerequisites for the formation of epithelial tissues, we have investigated the structure of the tight junctions in these cultures. We have found two types of cellular organization in the culture: monolayers and semi-spherical clusters. Electron microscopy has revealed tight junctions near the apical region of the lateral membranes between cells in the monolayers and cells at the surface of the clusters. The cells in the interior of the clusters also have tight junctions and are organized around a central lumen. These interior cells retain more secretory granules than the surface or monolayer cells, suggesting that they maintain their original character as acinar cells. The synthesis of claudin-4 increases during culture, although it is not detectable in the cells immediately after isolation from the glands. Immunofluorescence microscopy has shown that claudin-4 is synthesized in the monolayers and at the surface of the clusters, but not inside the clusters. Only claudin-3, which is present in the original acinar cells following isolation and in the intact gland, has been detected inside the clusters. These results suggest that differences in claudin expression are related to the three-dimensional structures of the cell cultures and reflect their ability to function as acinar cells.
机译：紧密连接对于维持上皮细胞极性至关重要。我们以前已经建立了唾液腮腺腺泡细胞的原代培养系统，该系统保留了它们产生新的分泌颗粒和以信号依赖方式分泌蛋白质的能力。因为细胞极性和细胞间粘附是上皮组织形成的前提，所以我们研究了这些培养物中紧密连接的结构。我们在文化中发现了两种类型的细胞组织：单层和半球形簇。电子显微镜显示单层细胞与簇表面细胞之间的侧膜顶部附近紧密连接。簇内部的细胞也有紧密的连接，并围绕中央管腔组织。这些内部细胞比表面或单层细胞保留更多的分泌颗粒，表明它们保持了腺泡细胞的原始特征。 claudin-4的合成在培养过程中增加，尽管从腺体分离后在细胞中无法立即检测到。免疫荧光显微镜显示claudin-4是在单层和簇表面但不在簇内部合成的。仅在分离后的原始腺泡细胞中和完整的腺体中存在claudin-3。这些结果表明claudin表达的差异与细胞培养物的三维结构有关，并反映了它们作为腺泡细胞的功能。

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  • 来源
    《Cell and Tissue Research》 |2007年第1期|59-70|共12页
  • 作者

    Bing Qi; Junko Fujita-Yoshigaki; Hiromi Michikawa; Keitaro Satoh; Osamu Katsumata; Hiroshi Sugiya;

  • 作者单位

    Department of Physiology Nihon University School of Dentistry at Matsudo Sakaecho-nishi 2-870-1 Matsudo Chiba 271-8587 Japan;

    Department of Physiology Nihon University School of Dentistry at Matsudo Sakaecho-nishi 2-870-1 Matsudo Chiba 271-8587 Japan;

    Department of Physiology Nihon University School of Dentistry at Matsudo Sakaecho-nishi 2-870-1 Matsudo Chiba 271-8587 Japan;

    Department of Physiology Nihon University School of Dentistry at Matsudo Sakaecho-nishi 2-870-1 Matsudo Chiba 271-8587 Japan;

    Department of Physiology Nihon University School of Dentistry at Matsudo Sakaecho-nishi 2-870-1 Matsudo Chiba 271-8587 Japan;

    Department of Physiology Nihon University School of Dentistry at Matsudo Sakaecho-nishi 2-870-1 Matsudo Chiba 271-8587 Japan;

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  • 正文语种 eng
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  • 关键词

    Tight junction; Claudin; Parotid acinar cell; Primary culture; Secretory granule; Rat (Sprague Dawley; male);

    机译：紧密连接;克劳丁;腺泡细胞;原代培养;分泌颗粒;大鼠（Sprague Dawley;雄性）;

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