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首页> 外文期刊>Cell and Tissue Research >Expression of PU.1 and terminal differentiation of alveolar macrophages in newborn rats
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Expression of PU.1 and terminal differentiation of alveolar macrophages in newborn rats

机译：新生大鼠PU.1的表达及肺泡巨噬细胞的终末分化

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摘要

PU.1, which is a transcription factor, promotes the terminal differentiation of alveolar macrophages (AMs). Its expression is regulated by granulocyte/macrophage colony-stimulating factor (GM-CSF). In this study of AMs in newborn rats, we performed immunohistochemical staining, acid phosphatase staining, reverse transcriptase polymerase chain reaction (RT-PCR), quantitative real-time PCR, cytokine assay, and electron microscopy. AMs at 3 and 7 days after birth had a large foamy appearance with an intracytoplasmic accumulation of surfactants. Weak expression of PU.1 was observed in the nuclei. AMs at 15 days after birth were smaller, and PU.1 expression had increased. Ultrastructurally, AMs at 1 day after birth had a smooth surface and abundant lamellar structures in the cytoplasm, whereas AMs at 56 days after birth were characterized by (1) abundant microvillar projections on the cell surface, and (2) well-developed lysosomes and a few lamellar structures in the cytoplasm. Acid phosphatase activity and the expression of mannose receptor, scavenger receptor, and GM-CSF receptor α were enhanced in AMs with time after birth. These results suggest that AMs are initially immature, and that their terminal differentiation starts after birth concomitantly with an increased expression of PU.1.
机译：PU.1是一种转录因子，可促进肺泡巨噬细胞（AM）的终末分化。其表达受粒细胞/巨噬细胞集落刺激因子（GM-CSF）调节。在这项对新生大鼠AM的研究中，我们进行了免疫组织化学染色，酸性磷酸酶染色，逆转录酶聚合酶链反应（RT-PCR），实时定量PCR，细胞因子测定和电子显微镜检查。出生后第3天和第7天的AMs具有较大的泡沫外观，并在细胞质内积累了表面活性剂。在细胞核中观察到PU.1的弱表达。出生后15天的AM较小，PU.1表达增加。在超微结构上，出生后1天的AM具有光滑的表面和丰富的层状结构，而出生后56天的AM具有以下特征：（1）细胞表面有大量微绒毛突起，（2）发达的溶酶体和细胞质中的一些层状结构。出生后随着年龄的增长，酸性磷酸酶活性以及甘露糖受体，清道夫受体和GM-CSF受体α的表达均增强。这些结果表明AMs最初是不成熟的，并且它们的终末分化在出生后就开始，并伴随着PU.1的表达增加。

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  • 来源
    《Cell and Tissue Research》 |2007年第1期|71-79|共9页
  • 作者

    Haruko Iwabuchi; Takashi Kawasaki; Takashi Yamamoto; Makoto Uchiyama; Koh Nakata; Makoto Naito;

  • 作者单位

    Division of Cellular and Molecular Pathology Department of Cellular Function Niigata University Graduate School of Medical and Dental Sciences Asahimachi 1-757 Niigata 951-8510 Japan;

    Division of Cellular and Molecular Pathology Department of Cellular Function Niigata University Graduate School of Medical and Dental Sciences Asahimachi 1-757 Niigata 951-8510 Japan;

    Division of Cellular and Molecular Pathology Department of Cellular Function Niigata University Graduate School of Medical and Dental Sciences Asahimachi 1-757 Niigata 951-8510 Japan;

    Division of Pediatrics Department of Homeostatic Regulation and Development Niigata University Graduate School of Medical and Dental Sciences Niigata Japan;

    Bioscience and Medical Research Center Niigata University Medical and Dental Hospital Niigata Japan;

    Division of Cellular and Molecular Pathology Department of Cellular Function Niigata University Graduate School of Medical and Dental Sciences Asahimachi 1-757 Niigata 951-8510 Japan;

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  • 正文语种 eng
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  • 关键词

    PU.1; Alveolar macrophage; GM-CSF; Surfactant; Rat (Wistar; newborn);

    机译：PU.1;肺泡巨噬细胞;GM-CSF;表面活性剂;大鼠（Wistar;新生儿）;

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