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首页> 外文期刊>Cell and Tissue Research >Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres
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Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres

机译：视网膜PACAP纤维支配的大鼠间质小叶脑啡肽神经元中c-Fos的光诱导

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摘要

The brain’s biological clock, located in the suprachiasmatic nucleus (SCN), is synchronised with the cyclic environment by photic and non-photic cues. Photic information to the SCN is mediated by pituitary adenylate-cyclase-activating polypeptide (PACAP)-containing retinal ganglion cells (RGCs), whereas non-photic input originates primarily from neuropeptide Y (NPY) cells in the ipsilateral thalamic intergeniculate leaflet (IGL). RGCs also seem to project to the IGL, indicating a role for this structure in the integration of photic and non-photic inputs related to the resetting of the biological clock. In the present study, we have used anterograde tracing from both eyes, bilateral eye enucleation, double-immunofluorescence histochemistry, high-resolution confocal laser scanning microscopy and three-dimensional computer analysis to show that (1) PACAP-containing RGCs project to the IGL and are the only source for the PACAP-immunoreactive fibres in the IGL; (2) a few NPY-containing neurons in the IGL are innervated by PACAP-containing retinal nerve fibres and the contacts are both axodendritic and axosomatic; (3) most enkephalin-immunoreactive neurons in the IGL are innervated by PACAP-containing retinal afferents and the contacts are mainly axodendritic; (4) light stimulation at various time points activates (as evidenced by c-Fos induction) enkephalin-positive neurons but not NPY-immunoreactive neurons. The findings suggest that PACAP-immunoreactive retinal afferents in the IGL primarily innervate enkephalin-immunoactive neurons and that the enkephalin-containing neurons, which project locally and to the contralateral IGL, are activated by light independent of diurnal time.
机译：位于视交叉上核（SCN）的大脑生物钟通过光和非光信号与循环环境同步。 SCN的磷酸化信息是由含有垂体腺苷酸-环化酶激活多肽（PACAP）的视网膜神经节细胞（RGC）介导的，而非磷酸化的输入主要源自同侧丘脑间质小叶（IGL）中的神经肽Y（NPY）细胞。 。 RGC似乎也向IGL投射，表明该结构在与生物钟重置相关的光和非光输入的整合中的作用。在本研究中，我们使用了双眼的顺行性示踪，双眼摘除术，双免疫荧光组织化学，高分辨率共聚焦激光扫描显微镜和三维计算机分析，以显示（1）含PACAP的RGC投射至IGL并且是IGL中PACAP免疫反应纤维的唯一来源； （2）IGL中的一些含有NPY的神经元被含有PACAP的视网膜神经纤维支配，并且接触都是轴突和轴突。 （3）IGL中大多数脑啡肽免疫反应性神经元被含PACAP的视网膜传入神经支配，并且接触主要是轴突。 （4）在不同时间点的光刺激会激活脑啡肽阳性神经元（由c-Fos诱导证明），但不会激活NPY免疫反应性神经元。研究结果表明，IGL的PACAP免疫反应性视网膜传入神经主要支配脑啡肽免疫活性神经元，局部投射到对侧IGL的含脑啡肽神经元被光激活，与昼夜时间无关。

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