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首页> 外文期刊>Cell and Tissue Research >C/EBPα knock-in hepatocytes exhibit increased albumin secretion and urea production
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C/EBPα knock-in hepatocytes exhibit increased albumin secretion and urea production

机译：C /EBPα敲入的肝细胞显示白蛋白分泌增加和尿素产生

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摘要

The CCAAT/enhancer-binding protein α (C/EBPα) is essential for maintaining the differentiated state of hepatocytes in vivo. C/EBPα activates albumin transcription and coordinates the expression of multiple ornithine cycle enzymes involved in urea production. We have examined the effects of the C/EBPα knock-in gene on the mRNA and protein expression of genes involved in liver-specific functions, such as albumin and urea production. Albumin mRNA and protein levels are higher in knock-in hepatocytes compared with wild-type hepatocytes. mRNA levels for the ornithine cycle enzymes, namely arginase, carbamylphosphate synthetase 1, and ornithine transcarbamylase, also increase. Albumin secretion and urea production are sustained at higher levels in culture in knock-in hepatocytes. We have previously established that C/EBPα induces early liver glycogen storage in C/EBPα knock-in mice. Our present observations underline the importance of C/EBPα in liver metabolism and suggest that the induction of C/EBPα expression enhances hepatocyte function.
机译：CCAAT /增强子结合蛋白α（C /EBPα）对于维持体内肝细胞的分化状态至关重要。 C /EBPα激活白蛋白转录并协调尿素生产中涉及的多种鸟氨酸循环酶的表达。我们已经检查了C /EBPα敲入基因对参与肝脏特异性功能（例如白蛋白和尿素生产）的基因的mRNA和蛋白质表达的影响。与野生型肝细胞相比，敲入肝细胞的白蛋白mRNA和蛋白水平更高。鸟氨酸循环酶，即精氨酸酶，氨基甲酸酯磷酸合成酶1和鸟氨酸转氨甲酰酶的mRNA水平也增加。在敲入的肝细胞中，培养物中白蛋白的分泌和尿素的产生持续较高的水平。我们先前已经确定，C /EBPα会在C /EBPα敲入小鼠中诱导早期肝糖原存储。我们目前的观察结果强调了C /EBPα在肝脏代谢中的重要性，并表明C /EBPα表达的诱导可增强肝细胞功能。

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