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首页> 外文期刊>Cellular and Molecular Bioengineering >Effect of Surface Patterning and Presence of Collagen I on the Phenotypic Changes of Embryonic Stem Cell Derived Cardiomyocytes
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Effect of Surface Patterning and Presence of Collagen I on the Phenotypic Changes of Embryonic Stem Cell Derived Cardiomyocytes

机译：表面图案和胶原蛋白I的存在对胚胎干细胞衍生心肌细胞表型变化的影响

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摘要

Embryonic stem cell derived cardiomyocytes have been widely investigated for stem cell therapy or in vitro model systems. This study examines how two specific biophysical stimuli, collagen I and cell alignment, affect the phenotypes of embryonic stem cell derived cardiomyocytes in vitro. Three phenotypic indicators are assessed: sarcomere organization, cell elongation, and percentage of binucleation. Murine embryonic stem cells were differentiated in a hanging drop assay and cardiomyocytes expressing GFP-α-actinin were isolated by fluorescent sorting. First, the effect of collagen I was investigated. Addition of soluble collagen I markedly reduced binucleation as a result of an increase in cytokinesis. Laden with a collagen gel layer, myocyte mobility and cell shape change were impeded. Second, the effect of cell alignment by microcontact printing and nanopattern topography was investigated. Both patterning techniques induced cell alignment and elongation. Microcontact printing of 20 μm line pattern accelerated binucleation and nanotopography with 700 nm ridges and 3.5 μm grooves negatively regulated binucleation. This study highlights the importance of biophysical cues in the morphological changes of differentiated cardiomyocytes and may have important implications on how these cells incorporate into the native myocardium.
机译：胚胎干细胞衍生的心肌细胞已被广泛研究用于干细胞治疗或体外模型系统。这项研究检查了两种特定的生物物理刺激物（胶原蛋白I和细胞排列）如何在体外影响胚胎干细胞衍生的心肌细胞的表型。评估了三个表型指标：肌节组织，细胞伸长和双核百分比。在悬滴法中分化出小鼠胚胎干细胞，并通过荧光分选分离表达GFP-α-肌动蛋白的心肌细胞。首先，研究了胶原蛋白I的作用。由于胞质分裂增加，可溶性胶原蛋白I的加入显着减少了双核化。载有胶原蛋白凝胶层的肌细胞活动性和细胞形状改变受到阻碍。其次，研究了通过微接触印刷和纳米图案形貌进行细胞排列的效果。两种图案化技术均诱导细胞排列和伸长。微接触印刷的20μm线型图案可加速双核​​化和纳米形貌，并具有700 nm的脊和3.5μm的凹槽，从而负向调节双核化。这项研究强调了生物物理学提示在分化的心肌细胞形态变化中的重要性，并且可能对这些细胞如何整合到天然心肌中具有重要意义。

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