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Kinetic Characterization by Surface Plasmon Resonance-Based Biosensors: Principle and Emerging Trends

机译:基于表面等离子体共振的生物传感器的动力学表征:原理和新兴趋势。

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摘要

Within the last three decades, surface plasmon resonance (SPR)-based optical biosensors have emerged as leading instruments for the study of biomolecular interactions. The rapid dissemination of the technology has provided researchers with versatile and sensitive instruments for the identification of kinetic and thermodynamic parameters related to macromolecular interactions. This review article presents the basic principles, as well as the classical experimental approaches and data treatment techniques related to the use of commercially available instruments for the characterization of protein–protein interactions. Emphasis will be put on new experimental trends aiming at improving two important aspects of any SPR kinetic studies, i.e., proteins tethering on biosensor surfaces as well as the optimization of data collection.
机译:在过去的三十年中,基于表面等离子体共振(SPR)的光学生物传感器已经成为研究生物分子相互作用的主要手段。该技术的快速传播为研究人员提供了通用且灵敏的仪器,用于鉴定与大分子相互作用有关的动力学和热力学参数。这篇综述文章介绍了基本原理以及与使用商业化仪器表征蛋白质-蛋白质相互作用有关的经典实验方法和数据处理技术。将重点放在旨在改善任何SPR动力学研究的两个重要方面的新实验趋势上,即在生物传感器表面上束缚蛋白质以及优化数据收集。

著录项

  • 来源
    《Cellular and Molecular Bioengineering》 |2008年第4期|204-215|共12页
  • 作者单位

    Department of Chemical Engineering Institute of Biomedical Engineering Groupe de Recherche en Sciences et Technologies Biomédicales Ecole Polytechnique de Montréal P.O. Box 6079 Station Centre-ville Montreal QC H3C 3A7 Canada;

    Department of Chemical Engineering Institute of Biomedical Engineering Groupe de Recherche en Sciences et Technologies Biomédicales Ecole Polytechnique de Montréal P.O. Box 6079 Station Centre-ville Montreal QC H3C 3A7 Canada;

    Animal Cell Technology Group Bioprocess Sector Biotechnology Research Institute National Research Council Canada 6100 Royalmount avenue Montreal QC H4P 2R2 Canada;

    Department of Chemical Engineering Institute of Biomedical Engineering Groupe de Recherche en Sciences et Technologies Biomédicales Ecole Polytechnique de Montréal P.O. Box 6079 Station Centre-ville Montreal QC H3C 3A7 Canada;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    SPR; Global analysis; Kinetic titration series; Coiled-coil; Affinity capture;

    机译:SPR;全局分析;动力学滴定系列;卷取线圈;亲和捕获;

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