Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules

Yusuke Suga; Hirobumi Sunayama; Tooru Ooya; Toshifumi Takeuchi

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Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules

机译：使用蛋白质偶联的可裂解单体制备分子印迹聚合物，然后在特定位置进行荧光报道分子的印迹后印迹

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Molecularly imprinted polymers were prepared using a protein-conjugated disulfide cleavable monomer. After removing the protein by disulfide reduction, a thiol-reactive fluorophore was introduced into the thiol residue located only inside the Imprinted cavity, resulting in specific transduction of the binding events into fluorescence spectral change.

机译：使用蛋白质偶联的二硫键可裂解的单体制备分子印迹的聚合物。通过二硫化物还原去除蛋白质后，将硫醇反应性荧光团引入仅位于印迹腔内的硫醇残基中，从而将结合事件特异性转导为荧光光谱变化。

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《Chemical Communications》 |2013年第76期|8450-8452|共3页
作者
Yusuke Suga; Hirobumi Sunayama; Tooru Ooya; Toshifumi Takeuchi;
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作者单位

Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku,Kobe 657-8501, Japan;

Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku,Kobe 657-8501, Japan;

Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku,Kobe 657-8501, Japan;

Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku,Kobe 657-8501, Japan;

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1. Fluorescence signaling molecularly imprinted polymers for antibiotics prepared via site-directed post-imprinting introduction of plural fluorescent reporters within the recognition cavity [J] . Sunayama Hirobumi, Ohta Takeo, Kuwahara Atsushi, Journal of Materials Chemistry, B. materials for biology and medicine . 2016,第44期

机译：通过在识别腔内进行定点印后引入多个荧光报告分子制备的用于抗生素的荧光信号分子印迹聚合物
2. Site-specific post-imprinting modification of molecularly imprinted polymer nanocavities with a modifiable functional monomer for prostate cancer biomarker recognition [J] . Hiroki Matsumoto, Hirobumi Sunayama, Yukiya Kitayama, Science and technology of advanced materials . 2019,第1期

机译：特异性特异性后印刷分子印迹聚合物纳米遗传性与可修饰的官能单体进行前列腺癌生物标志物识别
3. Fluorescent protein recognition polymer thin films capable of selective signal transduction of target binding events prepared by molecular imprinting with a post-imprinting treatment [J] . Sunayama H., Ooya T., Takeuchi T. Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2010,第2期

机译：能够选择性信号转导目标结合事件的荧光蛋白识别聚合物薄膜，该薄膜是通过分子印迹和印迹后处理制备的
4. Polymer-based High Selective Protein Sensing System Prepared by Molecular Imprinting and Post-imprinting Modifications [C] . Hirobumi Sunayama, Toshifumi Takeuchi 日本化学会春季年会講演予稿集 . 2018

机译：基于聚合物的高选择性蛋白质传感系统，通过分子印迹制备和印记后修饰
5. Chemical sensors based on swelling polymer microspheres and uncrosslinked fluorescently-labelled molecularly imprinted polymers. [D] . Mwangi, George K. 2005

机译：基于膨胀的聚合物微球和未交联的荧光标记分子印迹聚合物的化学传感器。
6. Site-specific post-imprinting modification of molecularly imprinted polymer nanocavities with a modifiable functional monomer for prostate cancer biomarker recognition [O] . Hiroki Matsumoto, Hirobumi Sunayama, Yukiya Kitayama, 2019

机译：用可修饰的功能单体对分子印迹聚合物纳米腔进行位点特异性印迹后修饰以用于前列腺癌生物标记识别
7. Fluorescent Imprinted Polymers Prepared with 2-Acrylamidoquinoline as a Signaling Monomer [O] . -1

机译：用2-丙烯酰胺喹啉制备的荧光印迹聚合物作为信号传导单体

Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules

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