...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemical Research in Chinese Universities >Prokaryotic Expression, Purification, Antibody Production of a NH_2 -Terminal Fragment of mCLCA3 Protein and Analysis of mClCA3 Protein Expression in Asthmatic Mouse Lung
【24h】

Prokaryotic Expression, Purification, Antibody Production of a NH_2 -Terminal Fragment of mCLCA3 Protein and Analysis of mClCA3 Protein Expression in Asthmatic Mouse Lung

机译：mCLCA3蛋白NH_2末端片段的原核表达，纯化，抗体生产以及哮喘小鼠肺中mClCA3蛋白的表达分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

mCLCA3 is a member of calcium activated chloride channel ( CACC) family that may play an important role in mucin packaging and secretion in asthmatic and cystic fibrosis lung. To study the protein structure and expression of mCLCA3 in asthmatic mouse lung, an N-terminal 269 amino acid peptide of mCLCA3 was expressed in E. coli, purified to homogeneity and rabbit polyclonal antibodies against this peptide were generated. Immunohistochemistry of asthmatic mouse lung using the antibody indicated exclusive mCLCA3 expression in mucin granules of goblet cells in airway surface and lumen. Immunoblot analysis of lavage fluid from asthmatic mouse lung revealed a single 90 kDa protein form of mClCA3. The results demonstrate that the 90 kDa N-terminal peptide, neither the full-length protein nor the reported N-terminal 35 kDa cleaved form of mClCA3 is the major functional form involved in the packaging and exocytosis of mucin granules in asthmatic goblet cells.
机译：mCLCA3是钙激活的氯离子通道（CACC）家族的成员，在哮喘和囊性纤维化肺的粘蛋白包装和分泌中可能起重要作用。为了研究哮喘小鼠肺中mCLCA3的蛋白质结构和表达，在大肠杆菌中表达了mCLCA3的N端269个氨基酸肽，纯化至均一，并产生了针对该肽的兔多克隆抗体。使用该抗体的哮喘小鼠肺部免疫组织化学表明，mCLCA3在气道表面和管腔的杯状细胞黏蛋白颗粒中仅表达。哮喘小鼠肺灌洗液的免疫印迹分析表明，mClCA3具有90 kDa的蛋白质形式。结果表明，90 kDa N末端肽，既不是全长蛋白也不是mClCA3的N末端35 kDa裂解形式，是参与哮喘杯状细胞粘蛋白颗粒包装和胞吐作用的主要功能形式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号