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首页> 外文期刊>Chemosphere >Mechanisms of strontium's adsorption by Saccharomyces cerevisiae: Contribution of surface and intracellular uptakes
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Mechanisms of strontium's adsorption by Saccharomyces cerevisiae: Contribution of surface and intracellular uptakes

机译：酿酒酵母吸附锶的机制：表面和细胞内摄取的贡献

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The objective of this work was to explore the mechanisms participating in strontium sorption by living Saccharomyces cerevisiae (S. cerevisiae). The location of strontium adsorbed by S. cerevisiae was studied by our plasmolysis treatment. The contribution of physical and chemical mechanisms was determined quantitatively by desorption and blockage of functional groups. Moreover, our results indicated that bioaccumulation also played a major role in biosorption by living cells. Thus, supplementary methods including 2-DE (two-dimensional electrophoresis) and Matrix-Assisted Laser Desorption/lonization Tandem Time of Flight Mass Spectrometry (MALDI-TOF-TOF) were employed to analyze the different proteins.The subsequent desorption % of Sr2+ by Distilled Water (DW), NH4NO3 and EDTA-Na-2 from Sr2+ loaded sorbents indicated a minor role for physical adsorption, while ion exchange and complexation were responsible for approximately 20% and 40%. Specific blockage of functional groups revealed that carboxyl and amine groups played an important role in SP2+ binding to the living S. cerevisiae. From our MALDITOF-TOF results, we concluded that 38 proteins showed up-regulated expression profiles and 11 proteins showed down-regulated after biosorption. Moreover, proteins belong to: phagocytic function (Act1p); ion channel (S-adenosylmethionine synthase); glycolysis (Tubulin) may directly involve in strontium bioaccumulation.In conclusion, the present work indicates that the strontium sorption mechanism by living S. cerevisiae is complicated including ion-exchange along with complexation as the main mechanism, whereas the other mechanisms such as physical adsorption play a minor contribution. Metabolically-dependent proteins may play an important role in bioaccumulation. (C) 2018 Elsevier Ltd. All rights reserved.
机译：这项工作的目的是探讨活酿酒酵母（S. cerevisiae）参与锶吸附的机制。通过我们的溶菌处理研究了酿酒酵母吸附的锶的位置。物理和化学机制的贡献是通过官能团的解吸和封闭来定量确定的。此外，我们的结果表明，生物积累在活细胞的生物吸附中也起着重要作用。因此，采用了包括2-DE（二维电泳）和基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF-TOF）在内的补充方法来分析不同的蛋白质。载有Sr2 +的吸附剂的蒸馏水（DW），NH4NO3和EDTA-Na-2对物理吸附的作用很小，而离子交换和络合作用约占20％和40％。特定的官能团封闭表明，羧基和胺基在SP2 +与酿酒酵母的结合中起着重要作用。根据我们的MALDITOF-TOF结果，我们得出结论，生物吸附后38种蛋白质显示出表达上调，11种蛋白质显示下调。而且，蛋白质属于：吞噬功能（Act1p）；离子通道（S-腺苷甲硫氨酸合酶）；糖酵解（Tubulin）可能直接参与锶的生物富集。总之，目前的工作表明，活的酿酒酵母对锶的吸附机理很复杂，包括离子交换和络合为主要机理，而其他机理如物理吸附。发挥很小的作用。代谢依赖性蛋白可能在生物蓄积中起重要作用。 （C）2018 Elsevier Ltd.保留所有权利。

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