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首页> 外文期刊>Chemosphere >Hydrogen sulfide exposure induces jejunum injury via CYP450s/ROS pathway in broilers
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Hydrogen sulfide exposure induces jejunum injury via CYP450s/ROS pathway in broilers

机译：暴露于硫化氢通过肉仔鸡中的CYP450s / ROS途径诱导空肠损伤

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摘要

Hydrogen sulfide (H2S) is generally recognized as a highly poisonous environmental and industrial pollutant. Previous toxicological studies of H2S are mainly focused on the nervous and cardiovascular system. There are few reports on the H2S toxicity effects on jejunum to our knowledge. Our study examined the morphological changes and antioxidant functions of broiler jejunum after the 42-day exposure to H2S. Effects of H2S on morphological damage and immune function in the broiler jejunum were analyzed from the perspective of CYP450s and oxidative stress via transcriptomics and quantitative real-time PCR (qRT-PCR). It was found that the activities of GPx, CAT, SOD, and T-AOC and the level of GSH were observably decreased (P 0.05), while the contents of MDA and H2O2 were remarkably increased (P 0.05) in the jejunums of broilers exposed to H2S, which undergone a process of oxidative stress, and typical inflammatory changes and apoptosis could be observed. Transcriptional profiling results showed that 208 genes were significantly up-regulated while 295 genes were remarkably down regulated in H2S group. The expression of CYP450s, inflammation and apoptosis-related genes were also significantly increased. In conclusion, H2S led to the redox homeostasis disorder through CYP450s differential expression in broiler jejunum. The jejunal inflammatory response, apoptosis along with the immune dysfunction were subsequently observed, which eventually caused jejunal morphology and functional damage. The present study further enriches and perfects the mechanism theory of H2S toxicity on broilers, which may be valuable for the risk assessment of H2S and human health protection. (C) 2018 Elsevier Ltd. All rights reserved.
机译：硫化氢（H2S）通常被认为是剧毒的环境和工业污染物。以前H2S的毒理学研究主要集中在神经和心血管系统。据我们所知，几乎没有关于H2S对空肠的毒性作用的报道。我们的研究检查了肉鸡空肠暴露于H2S 42天后的形态变化和抗氧化功能。通过转录组学和定量实时荧光定量PCR（qRT-PCR），从CYP450s和氧化应激的角度分析了H2S对肉鸡空肠形态损伤和免疫功能的影响。结果发现，空肠中GPx，CAT，SOD和T-AOC的活性和谷胱甘肽水平显着降低（P <0.05），而MDA和H2O2的含量显着增加（P <0.05）。暴露于H2S的肉鸡经历了氧化应激过程，并观察到典型的炎症变化和细胞凋亡。转录谱分析结果表明，H2S组的208个基因显着上调，而295个基因的显着下调。 CYP450s的表达，炎症和凋亡相关基因也明显增加。总之，H2S通过肉鸡空肠中CYP450s的差异表达导致氧化还原稳态障碍。随后观察到空肠炎性反应，细胞凋亡以及免疫功能障碍，最终导致空肠形态和功能损害。本研究进一步丰富和完善了硫化氢对肉鸡毒性的机理理论，对硫化氢的风险评估和人体健康保护可能具有重要的参考价值。 （C）2018 Elsevier Ltd.保留所有权利。

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  • 来源
    《Chemosphere》 |2019年第1期|25-34|共10页
  • 作者

    Zheng Shufang; Jin Xi; Chen Menghao; Shi Qunxiang; Zhang Hongfu; Xu Shiwen;

  • 作者单位

    Northeast Agr Univ, Coll Vet Med, Harbin 150030, Heilongjiang, Peoples R China;

    Northeast Agr Univ, Coll Vet Med, Dept Heilongjiang Common Anim Dis Prevent & Treat, Key Lab Prov Educ, Harbin 150030, Heilongjiang, Peoples R China;

    Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Hydrogen sulfide; Transcriptomics; Jejunum; CYP450s; Injury;

    机译：硫化氢;转录组学;空肠;CYP450s;损伤;

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