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首页> 外文期刊>Chemosphere >Hypoxia drives apoptosis independently of p53 and metallothionein transcript levels in hemocytes of the whiteleg shrimp Litopenaeus vannamei
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Hypoxia drives apoptosis independently of p53 and metallothionein transcript levels in hemocytes of the whiteleg shrimp Litopenaeus vannamei

机译：低氧驱动白腿虾南美白对虾血细胞中的p53和金属硫蛋白转录水平独立地导致细胞凋亡

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摘要

The cellular mechanisms used by the shrimp Litopenaeus vannamei to respond to hypoxia have been studied from the energetic metabolism and antioxidant angles. We herein investigated the participation of p53 and metallothionein (MT) in the apoptotic process in response to hypoxia in shrimp hemocytes. The Lvp53 or LvMT genes were efficiently silenced by injection of double stranded RNA for p53 or MT. The effects of silencing on apoptosis were measured as caspase-3 activity and flow cytometry in hemocytes after 24 and 48 h of hypoxia (1.5 mg DO L-1). Hemocytes from unsilenced animals had significantly higher apoptosis levels upon both times of hypoxia. The apoptotic levels were diminished but not suppressed in dsp53-silenced but not dsMT-silenced hemocytes after 24 h of hypoxia, indicating a contribution of Lvp53 to apoptosis. Apoptosis in normoxia was significantly higher in dsp53-and dsMT-silenced animals compared to the unsilenced controls, pointing to a possible cytoprotective role of LvMT and Lvp53 during the basal apoptotic program in normoxia. Overall, these results indicate that hypoxia augments apoptosis in shrimp hemocytes and high mRNA levels of Lvp53 and LvMT are not necessary for this response. (C) 2016 Elsevier Ltd. All rights reserved.
机译：从能量代谢和抗氧化剂的角度研究了对虾凡纳滨对虾低氧反应的细胞机制。我们在本文中研究了p53和金属硫蛋白（MT）在对虾血细胞缺氧的凋亡过程中的参与。 Lvp53或LvMT基因通过注射p53或MT的双链RNA有效地沉默。在缺氧24和48 h（1.5 mg DO L-1）后，通过沉默caspase-3活性和流式细胞仪测量沉默对凋亡的影响。在两次缺氧时，来自沉默动物的血细胞均具有明显更高的凋亡水平。缺氧24小时后，在dsp53沉默但在dsMT沉默的血细胞中凋亡水平降低，但没有被抑制，表明Lvp53对细胞凋亡的贡献。与未沉默的对照组相比，dsp53和dsMT沉默的动物在正常氧中的凋亡明显更高，这表明在正常氧的基础凋亡程序中，LvMT和Lvp53可能具有细胞保护作用。总体而言，这些结果表明，低氧增加了虾血细胞的凋亡，而Lvp53和LvMT的高mRNA水平对于该反应不是必需的。 （C）2016 Elsevier Ltd.保留所有权利。

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  • 来源
    《Chemosphere》 |2016年第10期|454-462|共9页
  • 作者

    Felix-Portillo Monserrath; Martinez-Quintana Jose A.; Arenas-Padilla Marina; Mata-Haro Veronica; Gomez-Jimenez Silvia; Yepiz-Plascenci Gloria;

  • 作者单位

    Ctr Invest Alimentac & Desarrollo, AC POB 1735 Carretera Ejido Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico;

    Univ Automoma Chihuahua, Fac Zootecnia & Ecol, Perifer Francisco R Almada,Km 1, Chihuahua 33820, Chihuahua, Mexico;

    Ctr Invest Alimentac & Desarrollo, AC POB 1735 Carretera Ejido Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico;

    Ctr Invest Alimentac & Desarrollo, AC POB 1735 Carretera Ejido Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico;

    Ctr Invest Alimentac & Desarrollo, AC POB 1735 Carretera Ejido Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico;

    Ctr Invest Alimentac & Desarrollo, AC POB 1735 Carretera Ejido Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Hypoxia; Apoptosis; Caspases; dsRNA silencing; p53; Litopenaeus vannamei;

    机译：缺氧;凋亡;胱天蛋白酶;dsRNA沉默;p53;南美白对虾;

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