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首页> 外文期刊>Chemosphere >Enhanced degradation of 2-nitrotoluene by immobilized cells of Micrococcus sp. strain SMN-1
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Enhanced degradation of 2-nitrotoluene by immobilized cells of Micrococcus sp. strain SMN-1

机译:Micrococcus sp。固定化细胞增强了2-硝基甲苯的降解。 SMN-1菌株

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Nitrotoluenes are the toxic pollutants of the environment because of their large scale use in the production of explosives. Biodegradation of such chemicals by microorganisms may provide an effective method for their detoxification. We have studied the degradation of 2-nitrotoluene by cells of Micrococcus sp. strain SMN-1 immobilized in various matrices such as polyurethane foam (PUF), sodium alginate (SA), sodium alginate-polyvinyl alcohol (SA-PVA), agar and polyacrylamide. The rate of degradation of 15 and 30 mM 2-nitrotoluene by freely suspended cells and immobilized cells in batches and fed-batch with shaken cultures were compared. The PUF-immobilized cells achieved higher degradation of 15 and 30 mM 2-nitrotoluene than freely suspended cells and the cells immobilized in SA-PVA, polyacrylamide, SA and agar. The PUF-immobilized cells could be reused more than 24 cycles without loosing their degradation capacity and showed more tolerance to pH and temperature changes than freely suspended cells. These results revealed the enhanced rate of degradation of 2-nitrotoluene by PUF-immobilized cells of Micrococcus sp. strain SMN-1.
机译:硝基甲苯是环境的有毒污染物,因为它们在炸药生产中被大量使用。微生物对此类化学物质进行生物降解可为它们的解毒提供有效的方法。我们已经研究了Micrococcus sp。的细胞对2-硝基甲苯的降解。菌株SMN-1固定在各种基质中,例如聚氨酯泡沫(PUF),藻酸钠(SA),藻酸钠-聚乙烯醇(SA-PVA),琼脂和聚丙烯酰胺。比较了自由悬浮的细胞和固定的细胞在分批培养和摇动培养的分批补料中降解15和30 mM 2-硝基甲苯的速率。固定有PUF的细胞比自由悬浮的细胞和固定在SA-PVA,聚丙烯酰胺,SA和琼脂中的细胞具有更高的15和30 mM 2-硝基甲苯降解能力。与自由悬浮的细胞相比,固定有PUF的细胞可以重复使用超过24个循环,而不会降低其降解能力,并且对pH和温度变化的耐受性更高。这些结果揭示了通过PUF固定化的Micrococcus sp。的细胞提高了2-硝基甲苯的降解速率。菌株SMN-1。

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