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首页> 外文期刊>Chemosphere >Mechanistic toxicity study of perfluorooctanoic acid in zebrafish suggests mitochondrial dysfunction to play a key role in PFOA toxicity
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Mechanistic toxicity study of perfluorooctanoic acid in zebrafish suggests mitochondrial dysfunction to play a key role in PFOA toxicity

机译：斑马鱼全氟辛酸的机械毒性研究表明，线粒体功能障碍在PFOA毒性中起关键作用

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摘要

The aquatic environment is an important site for perfluorooctanoic acid (PFOA) deposit. Nevertheless, the exact mode of action and its resulting toxicological effects in aquatic organisms remain largely unknown. To gain a better understanding of the mode of action of teleost PFOA toxicity, transcriptomics, proteo-mics, biochemical parameters and reproduction were integrated in this study. Male and female zebrafish were exposed to nominal concentrations of 0.1, 0.5 and 1 mg L~(-1) PFOA for 4 and 28 d resulting in PFOA accumulation which was higher in males than in females. These gender-related differences were likely caused by different elimination rates due to distinct hormone levels and differences in transport activity by solute carriers. The general mode of action of PFOA was described as an increase of the mitochondrial membrane permeability followed by an impairment of aerobic ATP production. Depletion of liver glyco-gen stores together with altered expression levels of transcripts involved in carbohydrate metabolism, with emphasis on anaerobic metabolism, was probably a means of compensating for this decreased aerobic efficiency. The mitochondrial dysfunction further resulted in effects on oxidative stress and apopto-sis at the gene transcript and protein level. As a consequence, evidence for the replacement of the affected cells and organelles to sustain tissue homeostasis was found at the transcript level, resulting in an even greater glycogen depletion. Despite this increase in metabolic expenditure, no effects on reproduction were found indicating that the fish seemed to cope with exposure to the tested concentrations of PFOA during the exposure period of 1 month.
机译：水生环境是全氟辛酸（PFOA）沉积的重要场所。然而，在水生生物中确切的作用方式及其所产生的毒理学作用仍然是未知的。为了更好地了解硬骨鱼PFOA毒性的作用方式，本研究整合了转录组学，蛋白质组学，生化参数和繁殖。雄性和雌性斑马鱼分别暴露于0.1、0.5和1 mg L〜（-1）PFOA的标称浓度达4和28 d，导致雄性比雌性中PFOA积累更高。这些与性别有关的差异可能是由于不同的激素水平和溶质携带者的转运活性差异而导致的消除率不同所致。 PFOA的一般作用方式被描述为线粒体膜通透性增加，然后有氧ATP产生受到损害。肝糖原存储的耗竭以及与碳水化合物代谢有关的转录物表达水平的改变，尤其是厌氧代谢，可能是弥补这种有氧效率下降的一种手段。线粒体功能障碍进一步导致在基因转录物和蛋白质水平上对氧化应激和凋亡的影响。结果，在转录本水平上发现了替换受影响的细胞和细胞器以维持组织稳态的证据，从而导致更大的糖原消耗。尽管新陈代谢支出有所增加，但未发现对繁殖的影响，表明该鱼似乎可以在1个月的暴露期中暴露于测试浓度的PFOA。

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  • 来源
    《Chemosphere》 |2013年第6期|844-856|共13页
  • 作者

    A. Hagenaars; L. Vergauwen; D. Benoot; K. Laukens; D. Knapen;

  • 作者单位

    Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Anrwerpen, Belgium;

    Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Anrwerpen, Belgium;

    Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Anrwerpen, Belgium;

    Biomedical Informatics Research Center Antwerpen (Biomina), Department of Mathematics and Computer Science, University of Antwerp, Middelheimlaan 1,2020 Antwerp, Belgium;

    Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Anrwerpen, Belgium ,Gamete Research Center (GRC), Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    PFOA; perfluorinated compounds; zebrafish; transcriptomics; proteomics; mitochondrial dysfunction;

    机译：PFOA;全氟化合物;斑马鱼转录组学蛋白质组学线粒体功能障碍;

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