...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemosphere >Evaluation of phenanthrene toxicity on earthworm (Eisenia fetida): An ecotoxicoproteomics approach
【24h】

Evaluation of phenanthrene toxicity on earthworm (Eisenia fetida): An ecotoxicoproteomics approach

机译：菲对E（Eisenia fetida）的毒性评价：生态毒理学方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The goal of this study was to identify promising new biomarkers of phenanthrene by identifying differentially expressed proteins in Eisenia fetida after exposure to phenanthrene. Extracts of earthworm epithelium collected at days 2, 7, 14, and 28 after phenanthrene exposure were analyzed by two dimensional electrophoresis (2-DE) and quantitative image analysis. Comparing the intensity of protein spots, 36 upregulated proteins and 45 downregulated proteins were found. Some of the downregulated and upreg-ulated proteins were verified by MALDI-TOF/TOF-MS and database searching. Downregulated proteins in response to phenanthrene exposure were involved in glycolysis, energy metabolism, chaperones, prote-olysis, protein folding and electron transport. In contrast, oxidation reduction, oxygen transport, defense systems response to pollutant, protein biosynthesis and fatty acid biosynthesis were upregulated in phenanthrene-treated E. fetida. In addition, ATP synthase b subunit, lysenin-related protein 2, lombricine kinase, glyceraldehyde 3-phosphate dehydrogenase, actinbinding protein, and extracellular globin-4 seem to be potential biomarkers since these biomarker were able to low levels (2.5 mg kg~(-1)) of phenanthrene. Our study provides a functional profile of the phenanthrene-responsive proteins in earthworms. The variable levels and trends in these spots could play a potential role as novel biomarkers for monitoring the levels of phenanthrene contamination in soil ecosystems.
机译：这项研究的目的是通过鉴定暴露于菲后的埃塞俄比亚fetida中差异表达的蛋白质来鉴定有前途的菲生物标志物。通过二维电泳（2-DE）和定量图像分析分析了菲暴露后第2、7、14和28天收集的worm上皮提取物。比较蛋白质斑点的强度，发现了36​​种上调的蛋白质和45种下调的蛋白质。通过MALDI-TOF / TOF-MS和数据库搜索验证了一些下调和上调的蛋白质。响应菲暴露而下调的蛋白质参与糖酵解，能量代谢，伴侣，蛋白水解，蛋白质折叠和电子转运。相反，在菲处理的大肠杆菌中，氧化还原，氧气输送，防御系统对污染物的反应，蛋白质的生物合成和脂肪酸的生物合成被上调。此外，ATP合酶b亚基，赖氨酸相关蛋白2，lombricine激酶，3-磷酸甘油醛脱氢酶，肌动蛋白结合蛋白和细胞外globin-4似乎是潜在的生物标志物，因为这些生物标志物的水平较低（2.5 mg kg〜（ -1））菲。我们的研究提供了the中菲响应蛋白的功能概况。这些斑点的可变水平和趋势可能作为监测土壤生态系统中菲污染水平的新型生物标志物发挥潜在作用。

著录项

  • 来源
    《Chemosphere》 |2013年第6期|963-971|共9页
  • 作者

    Shijin Wu; Xian Xu; Shiliang Zhao; Feichao Shen; Jianmeng Chen;

  • 作者单位

    College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China;

    College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China;

    College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China;

    College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China;

    College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Proteomics; Phenanthrene; Eisenia fetida; Biomarkers; MALDI-TOF MS/MS;

    机译：蛋白质组学;菲;et生物标志物;MALDI-TOF MS / MS;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号