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首页> 外文期刊>Chemosphere >Bioadsorption and bioaccumulation of chromium trivalent in Cr(Ⅲ)-tolerant microalgae: A mechanisms for chromium resistance
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Bioadsorption and bioaccumulation of chromium trivalent in Cr(Ⅲ)-tolerant microalgae: A mechanisms for chromium resistance

机译：三价铬在耐Cr（Ⅲ）的微藻中的生物吸附和生物富集：抗铬机理

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摘要

Anthropogenic activity constantly releases heavy metals into the environment. The heavy metal chromium has a wide industrial use and exists in two stable oxidation states: trivalent and hexavalent. While hexavalent chromium uptake in plant cells has been reported that an active process by carrying essential anions, the cation Cr(Ⅲ) appears to be taken up inactively. Dictyosphaerium chlorelloides (Dc1M), an unicellular green alga is a well-studied cell biological model organism. The present study was carried out to investigate the toxic effect of chromium exposures on wild-type Cr(Ⅲ)-sensitive (Dc1M~(wt)) and Cr(Ⅲ)-tol-erant (Dc1M~(Cr(Ⅲ)R30)) strains of these green algae, and to determine the potential mechanism of chromium resistance. Using cell growth as endpoint to determine Cr(Ⅲ)-sensitivity, the IC_(50(72)) values obtained show significant differences of sensitivity between wild type and Cr(Ⅲ)-tolerant cells. Scanning electron microscopy (SEM) showed significant morphological differences between both strains, such as decrease in cell size or reducing the coefficient of form; and transmission electron microscopy (TEM) revealed ultrastructural changes such as increased vacuolization and cell wall thickening in the Cr(Ⅲ)-tolerant strain with respect to the wild-type strain. Energy dispersive X-ray analysis (SEM/XEDS) revealed that Cr(Ⅲ)-tolerant D. chlorelloides cells are able to accumulate considerable amounts of chromium distributed in cell wall (bioadsorption) as well as in cytoplasm, vacuoles, and chloroplast (bio-accumulation). Morphological changes of Cr(Ⅲ)-tolerant D. chlorelloides cells and the presence of these electron-dense bodies in their cell structures can be understood as a Cr(Ⅲ) detoxification mechanism.
机译：人为活动不断将重金属释放到环境中。重金属铬具有广泛的工业用途，并且以两种稳定的氧化态存在：三价和六价。虽然已经报道了植物细胞中六价铬的吸收是一个通过携带必需阴离子而活跃的过程，但阳离子Cr（Ⅲ）却被吸收了。 Dictyosphaerium chlorelloides（Dc1M），一种单细胞绿藻是经过充分研究的细胞生物学模型生物。本研究旨在研究铬暴露对野生型Cr（Ⅲ）敏感（Dc1M〜（wt））和Cr（Ⅲ）耐受（Dc1M〜（Cr（Ⅲ）R30）的毒性作用。 ）这些绿藻的菌株，并确定抗铬的潜在机理。以细胞生长为终点确定Cr（Ⅲ）敏感性，得到的IC_（50（72））值表明野生型和Cr（Ⅲ）耐受细胞的敏感性存在显着差异。扫描电子显微镜（SEM）显示两种菌株之间存在明显的形态学差异，例如细胞大小的减少或形态系数的降低。透射电子显微镜（TEM）揭示了相对于野生型菌株，耐Cr（Ⅲ）菌株中超微结构的变化，如空泡增加和细胞壁增厚。能量色散X射线分析（SEM / XEDS）表明，耐Cr（Ⅲ）的D. chlorelloides细胞能够积累大量分布在细胞壁（生物吸附）以及细胞质，液泡和叶绿体中的铬（生物-积累）。 Cr（Ⅲ）耐受的小球藻D. chlorelloides细胞的形态变化和这些电子致密体的存在可以理解为Cr（Ⅲ）的解毒机理。

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  • 来源
    《Chemosphere》 |2013年第6期|1057-10581060-1063|共6页
  • 作者

    M. Pereira; M.C. Bartolome; S. Sanchez-Fortun;

  • 作者单位

    Department of Toxicology and Pharmacology, School of Veterinary Sciences, Complutense University, s Puerto de Hierro Ave., 28040 Madrid, Spain;

    School of Chemistry-Pharmacology, Michoacana de San Nicolas de Hidalgo University, 43 Santiago Tapia St., 58000 Morelia, Michoacan, Mexico;

    Department of Toxicology and Pharmacology, School of Veterinary Sciences, Complutense University, s Puerto de Hierro Ave., 28040 Madrid, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Dictyosphaerium chlorelloides; Cr(Ⅲ); Environmental adaptation; Toxicity; infrastructure; XEDS;

    机译：小球藻Cr（Ⅲ）;环境适应;毒性;基础设施;XEDS;

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