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首页> 外文期刊>Chemosphere >In vitro evaluation of cellular influences induced by stable fullerene C_(70) medium dispersion: Induction of cellular oxidative stress
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In vitro evaluation of cellular influences induced by stable fullerene C_(70) medium dispersion: Induction of cellular oxidative stress

机译：稳定富勒烯C_（70）介质分散诱导的细胞影响的体外评估：诱导细胞氧化应激

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摘要

Fullerene is one of the nanocarbons that is expected to have applications to life science, such as nanomed-icines. An understanding of the cellular influences of fullerene is essential for its application to life science. Although C_(60) and C_(70) are both known as major fullerenes, most previous reports about the cellular influences of fullerene are about C_(60). Thus we evaluated the cellular influences caused by C_(70). A stable and uniform C_(70)-medium dispersion was prepared. The dispersion was stable for the experimental period. Mitochondrial activity (MTT assay), colony forming ability (clonogenic assay), induction of oxidative stress (intracellular ROS and lipid peroxidation levels) and cellular uptake (TEM observation) in human keratinocyte HaCaT and lung carcinoma A549 cells exposed to C_(70) were examined. C_(70) did not influence mitochondrial activity. On the other hand, C_(70) dispersion inhibited colony formation at the concentration of 25.2 μg mL~(-1) Exposure to C_(70) dispersion caused an increase in intracellular ROS and lipid peroxidation levels. The induction of intracellular ROS level was inhibited by pre-treatment of the cells by antioxidants. TEM observations of C_(70) exposed cells showed cellular uptake of C_(70). These results were similar to the cellular influences caused by C_(60) which were reported by us previously. Although C_(70) did not cause cell death, it caused the induction of intracellular oxidative stress.
机译：富勒烯是一种有望应用于生命科学的纳米碳，例如纳米医学。对富勒烯的细胞影响的理解对于将其应用于生命科学至关重要。尽管C_（60）和C_（70）都被称为主要的富勒烯，但以前有关富勒烯的细胞影响的大多数报道都是关于C_（60）。因此，我们评估了由C_（70）引起的细胞影响。制备稳定且均匀的C_（70）-介质分散体。分散液在实验期间是稳定的。暴露于C_（70）的人角质形成细胞HaCaT和肺癌A549细胞的线粒体活性（MTT测定），集落形成能力（克隆形成测定），氧化应激诱导（细胞内ROS和脂质过氧化水平）和细胞摄取（TEM观察）检查。 C_（70）不影响线粒体活性。另一方面，在浓度为25.2μgmL〜（-1）时，C_（70）分散液抑制菌落形成。暴露于C_（70）分散液会导致细胞内ROS和脂质过氧化水平增加。抗氧化剂预处理可抑制细胞内ROS水平的诱导。 C_（70）暴露细胞的TEM观察显示细胞吸收C_（70）。这些结果类似于我们先前报道的C_（60）引起的细胞影响。尽管C_（70）不会引起细胞死亡，但它引起了细胞内氧化应激的诱导。

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  • 来源
    《Chemosphere》 |2013年第6期|1182-1188|共7页
  • 作者

    Masanori Horie; Keiko Nishio; Haruhisa Kato; Naohide Shinohara; Ayako Nakamura; Katsuhide Fujita; Shinichi Kinugasa; Shigehisa Endoh; Yasukazu Yoshida; Yoshihisa Hagihara; Hitoshi Iwahashi;

  • 作者单位

    Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Kitakyushu, Fukuoka 807-8555, Japan,Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, ikeda, Osaka 563-8577, Japan;

    Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, ikeda, Osaka 563-8577, Japan;

    National Metrology Institute of Japan (NMIJ), AIST, l-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan;

    Research Institute of Science for Safety and Sustainability (RISS), AIST, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan;

    National Metrology Institute of Japan (NMIJ), AIST, l-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan;

    Research Institute of Science for Safety and Sustainability (RISS), AIST, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan;

    National Metrology Institute of Japan (NMIJ), AIST, l-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan;

    Research Institute for Environmental Management Technology (EMTECH), AIST, Particle Measurement Group, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan;

    Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, ikeda, Osaka 563-8577, Japan;

    Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, ikeda, Osaka 563-8577, Japan;

    Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, ikeda, Osaka 563-8577, Japan,Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Fullerene; Oxidative stress; Cytotoxicity; Cellular uptake;

    机译：富勒烯;氧化应激细胞毒性;细胞摄取;

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