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首页> 外文期刊>Chemosphere >Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol
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Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol

机译：暴露于17α-炔雌醇的成年日本med（Oryzias latipes）的睾丸与卵巢相关的基因表达谱

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摘要

The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100 ng L~(-1) of 17α-ethinylestradiol (EE2) for 3-5 weeks, and 800 ng estradiol benzo-ate (EB) for 3 weeks (experiment 1), and a measured concentration of 20 ng L~(-1) EE2 for 1-6 weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4 weeks of exposure to 100 ng L~(-1) EE2, 3 weeks of exposure to 800 ng EB, and 6 weeks of exposure to 20 ng L~(-1) EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
机译：几种鱼类的睾丸（卵-卵）中卵母细胞的出现通常与雌激素化学物质的暴露有关。然而，睾丸卵子的诱导机制仍有待阐明。为了开发用于检测睾丸中睾丸卵的标记基因，将成年雄性med蝇暴露于标称浓度为100 ng L〜（-1）的17α-炔雌醇（EE2）3-5周，以及800 ng雌二醇苯甲酸酯（EB）进行3周（实验1），测量浓度为20 ng L〜（-1）EE2进行1-6周（实验II）。对睾丸进行组织学分析，并对睾丸，肝脏和大脑进行微阵列分析。在暴露于雌激素的medaka肝脏中进行的微阵列分析显示，卵黄蛋白原和绒毛膜促性腺激素是雌激素响应基因。暴露于100 ng L〜（-1）EE2 4周，暴露于800 ng EB 3周，暴露于20 ng L〜（-1）EE2 6周后，在睾丸中诱导出睾丸卵。暴露于雌激素的睾丸的微阵列分析显示与透明带（ZP）和卵母细胞标记基因42Sp50相关的基因上调。使用定量RT-PCR，我们证实了Zpc5基因可以用作检测男性中睾丸卵的标记。

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  • 来源
    《Chemosphere》 |2012年第7期|p.668-674|共7页
  • 作者

    Ikumi Hirakawa; Shinichi Miyagawa; Yoshinao Katsu; Yoshihiro Kagami; Norihisa Tatarazako; Tohru Kobayashi; Teruhiko Kusano; Takeshi Mizutani; Yukiko Ogino; Takashi Takeuchi; Yasuhiko Ohta; Taisen Iguchi;

  • 作者单位

    The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 751-8513, Japan,Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, the Graduate University for Advanced Studies, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan,Ecogenomics, Inc. Kurume, Fukuoka 839-0864, Japan;

    Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, the Graduate University for Advanced Studies, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan;

    Department of Biological Sciences, Hokkaido University, Sapporo 060-0810, Japan;

    Ecogenomics, Inc. Kurume, Fukuoka 839-0864, Japan;

    National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan;

    Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526, Japan;

    Ecogenomics, Inc. Kurume, Fukuoka 839-0864, Japan;

    Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, the Graduate University for Advanced Studies, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan;

    Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, the Graduate University for Advanced Studies, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan;

    The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 751-8513, Japan,Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Koyama, Tottori 680-8553, Japan;

    The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 751-8513, Japan,Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Koyama, Tottori 680-8553, Japan;

    Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, the Graduate University for Advanced Studies, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    medaka; 17α-ethinylestradiol; testis-ova; zona pellucida genes; microarray;

    机译：aka 17α-炔雌醇;睾丸卵透明带基因微阵列;

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