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首页> 外文期刊>Chemosphere >Responses to copper by the moss Plagiomnium cuspidatum: Hydrogen peroxide accumulation and the antioxidant defense system
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Responses to copper by the moss Plagiomnium cuspidatum: Hydrogen peroxide accumulation and the antioxidant defense system

机译：苔藓斜gio对铜的反应：过氧化氢的积累和抗氧化防御系统

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摘要

Using both histochemical and cytochemical methods, we investigated the effects of copper (Cu) on the production of hydrogen peroxide (H_2O_2) and superoxide anion (O_2~-) in the leaves of the moss Plagiomnium cuspidatum. Cu treatment significantly increased the contents of total thiobarbituric acid-reactive substances and H_2O_2, as well as the activity of guaiacol peroxidase and superoxide dismutase (SOD). Native PAGE detected all three forms of SOD (Mn-SOD, Fe-SOD and CuZn-SOD) in P. cuspidatum, and the increase in the total SOD activity appeared to be mainly caused by an increase in CuZn-SOD activity. According to cytochemical results, H_2O_2-dependent CeCl_3 precipitates were primarily localized in the plasma membranes and cell walls, and O_2~- was chiefly localized on the inner side of the plasma membrane and in the cytoplasm surrounding the chloroplasts. Experiments using imidazole as an inhibitor of NADPH oxidase, N-N-diethyldithiocarbamate as an inhibitor of CuZn-SOD, and 1,2-dihydroxyben-zene-3,5-disulphonic acid as an O_2~- scavenger indicated that a partial source of H_2O_2 in the cell walls may be NADPH oxidase. The results also showed that peroxidase (POD) is involved in the detoxification of H_2O_2. Increased POD activity induced by Cu may remove excess H_2O_2 caused by Cu.
机译：利用组织化学和细胞化学方法，我们研究了铜（Cu）对苔藓天竺葵叶片中过氧化氢（H_2O_2）和超氧阴离子（O_2〜-）产生的影响。铜处理显着增加了总硫代巴比妥酸反应性物质和H_2O_2的含量，以及愈创木酚过氧化物酶和超氧化物歧化酶（SOD）的活性。天然PAGE检测到虎杖中的三种SOD形式（Mn-SOD，Fe-SOD和CuZn-SOD），总SOD活性的增加似乎主要由CuZn-SOD活性的增加引起。根据细胞化学结果，依赖H_2O_2的CeCl_3沉淀主要位于质膜和细胞壁，而O_2〜-主要位于质膜的内侧和叶绿体周围的细胞质。使用咪唑作为NADPH氧化酶抑制剂，NN-二乙基二硫代氨基甲酸酯作为CuZn-SOD抑制剂以及1,2-二羟基苯-zene-3,5-二磺酸作为O_2〜-清除剂的实验表明，H_2O_2的部分来源细胞壁可以是NADPH氧化酶。结果还表明过氧化物酶（POD）参与H_2O_2的解毒。铜诱导的POD活性增加可能会去除铜引起的过量H_2O_2。

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  • 来源
    《Chemosphere》 |2009年第9期|1260-1265|共6页
  • 作者

    Yanfang Wu; Yahua Chen; Yanjun Yi; Zhenguo Shen;

  • 作者单位

    College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China;

    College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China;

    Qingdao Agricultural University, Qingdao 266109, PR China;

    College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    antioxidant enzyme; bryophyte; copper; hydrogen peroxide;

    机译：抗氧化酶苔藓植物铜;过氧化氢;

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