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首页> 外文期刊>Chemosphere >Characterization Of Heat Shock Protein 40 And 90 In Chironomus Riparius Larvae: Effects Of Di(2-ethylhexyl) Phthalate Exposure On Gene Expressions And Mouthpart Deformities
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Characterization Of Heat Shock Protein 40 And 90 In Chironomus Riparius Larvae: Effects Of Di(2-ethylhexyl) Phthalate Exposure On Gene Expressions And Mouthpart Deformities

机译：表征了Chironomus Riparius幼虫中热休克蛋白40和90：邻苯二甲酸二（2-乙基己基）酯对基因表达和口部畸形的影响

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Di(2-ethylhexyl) phthalate (DEHP) is a widely distributed phthalate that organisms are frequently exposed to due to its wide range of commercial and manufacturing uses as a plasticizer. Indeed, DEHP is often found in freshwater systems that receive domestic waste water discharges. Therefore, we evaluated the effects of DEHP on the mRNA levels of heat shock protein (HSP) 40 and 90 in chironomids. In addition, we evaluated the effects of exposure to DEHP on the induction of morphological deformities in chironomids. To accomplish this, partial sequences of HSP 40 and 90 from Chironomus riparius larvae were amplified and sequenced. The inferred amino acid sequences were then aligned with those of other insect HSP 40 and 90 genes. The results of this alignment revealed that there was a high degree of similarity among the homo-logues. In addition, the HSP 40 and 90 mRNA expressions were significantly upregulated in response to short and long-term exposure to DEHP at concentrations of 1,10, and 30 μg L~(-1). Furthermore, the occurrence of mouthpart deformities was significantly higher in chironomids that were treated with DEHP (12-20%) than in controls (3-5%). Taken together, these results indicate that HSP 40 and 90 play important roles in the physiological changes related to metabolism and cell protection that occur in C. riparius larvae that have been exposed to DEHP.
机译：邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种分布广泛的邻苯二甲酸酯，由于其广泛的商业用途和制造用途，可作为生物增塑剂。实际上，DEHP经常出现在接受生活污水排放的淡水系统中。因此，我们评估了DEHP对Chironomids中热休克蛋白（HSP）40和90 mRNA水平的影响。此外，我们评估了暴露于DEHP诱导的Chironomids形态畸变的影响。为了实现这一点，扩增了来自河鲈（Chironomus riparius）幼虫的HSP 40和90的部分序列并对其进行了测序。然后将推断的氨基酸序列与其他昆虫HSP 40和90基因的氨基酸序列进行比对。该比对的结果表明同系物之间高度相似。此外，在短期和长期暴露于浓度为1,10和30μgL〜（-1）的DEHP中，HSP 40和90 mRNA表达显着上调。此外，用DEHP治疗的手足动物口部畸形的发生率明显高于对照组（3-5％）（12-20％）。综上所述，这些结果表明，HSP 40和90在已暴露于DEHP的河豚草幼虫中发生的与代谢和细胞保护有关的生理变化中起重要作用。

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