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Microbial community structure analysis in a hybrid membrane bioreactor via high-throughput sequencing

机译:通过高通量测序在杂交膜生物反应器中的微生物群落结构分析

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摘要

Compared to the suspended culture (sludge) of a conventional bioreactor, hybrid membrane bioreactors (HMBRs) are more effective at removing pollutants owing to the addition of fillers addition of fillers that facilitate biofilm formation. However, the microbial community structure and composition in an HMBR remain unclear. We ran three laboratory-scale HMBRs at room temperature under different sludge retention times (SRTs; 10 d, 20 d, and 30 d) to compare the microbial diversity and community structure among the membrane surface, suspended filler surface, and mixed liquor using high-throughput sequencing. The results showed that SRT can markedly affect microbial community structure in the HMBR, and different trends appeared in the three functional units. The largest number of mutual operational taxonomic units was found in the activated sludge mixture and suspended carrier with an SRT of 20 d and 30 d. Species belonging to Proteobacteria in the 10 d SRT group had the greatest contribution to between-group differences. A longer SRT could mitigate membrane fouling by decreasing the relative abundance of Thauera and Sphaerotilus attached on the membrane surface. Comamonadaceae, a family of denitrifying bacteria, offer high denitrification potential for bioreactors operating under a long SRT.
机译:与常规生物反应器的悬浮培养(污泥)相比,由于添加促进生物膜形成的填料,杂种膜生物反应器(HMBRS)在除去污染物时更有效。然而,HMBR中的微生物群落结构和组合物仍然不清楚。我们在室温下在不同的污泥保留时间(SRTS; 10d,20d,30d)下进行三个实验室级HMBRS,以比较膜表面,悬浮填料表面的微生物多样性和群落结构,以及使用高的混合液 - 滴水排序。结果表明,SRT可以显着影响HMBR中的微生物群落结构,三种功能单元中出现不同的趋势。在活性污泥混合物和悬浮载体中发现最多的互操作分类单位,SRT为20d和30d。属于10 d SRT集团的植物的物种对组间差异的贡献最大。通过降低附着在膜表面上的粉末和斯坦氏菌菌,更长的SRT可以减轻膜污染。一种反硝化细菌的家族,番荔枝群,为在长时间的SRT下运行的生物反应器提供高脱氮电位。

著录项

  • 来源
    《Chemosphere》 |2021年第11期|130989.1-130989.8|共8页
  • 作者单位

    Hohai Univ Coll Environm Key Lab Integrated Regulat & Resource Dev Shallow Minist Educ Nanjing 210098 Peoples R China|Hohai Univ Coll Environm Nanjing 210098 Peoples R China|Xuzhou Univ Technol Sch Environm Engn Xuzhou 221018 Jiangsu Peoples R China;

    Hohai Univ Coll Environm Key Lab Integrated Regulat & Resource Dev Shallow Minist Educ Nanjing 210098 Peoples R China|Hohai Univ Coll Environm Nanjing 210098 Peoples R China;

    Hohai Univ Coll Environm Key Lab Integrated Regulat & Resource Dev Shallow Minist Educ Nanjing 210098 Peoples R China|Hohai Univ Coll Environm Nanjing 210098 Peoples R China;

    Xuzhou Univ Technol Sch Environm Engn Xuzhou 221018 Jiangsu Peoples R China;

    Xuzhou Univ Technol Sch Environm Engn Xuzhou 221018 Jiangsu Peoples R China;

    PLA Univ Sci & Technol Xuzhou 221000 Jiangsu Peoples R China;

    Xuzhou Univ Technol Sch Environm Engn Xuzhou 221018 Jiangsu Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Extracellularnbsp; polymeric substance; Microbial community structure; Biodiversity;

    机译:细胞外和NBSP;聚合物物质;微生物群落结构;生物多样性;

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