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首页> 外文期刊>The Chinese-German Journal of Clinical Oncology >Clinical Significance of p53, Proliferating Cell Nuclear Antigen and bcl-2 Expression in Bladder Transitional Cell Carcinoma
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Clinical Significance of p53, Proliferating Cell Nuclear Antigen and bcl-2 Expression in Bladder Transitional Cell Carcinoma

机译：膀胱移行细胞癌中p53，增殖细胞核抗原和bcl-2表达的临床意义

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Objective: To correlate the frequency of p53 mutations, bcl-2 expression and the proliferation status (proliferating cell nuclear antigen, PCNA) in patients with bladder cancer with cell proliferation, apoptosis and their clinico-pathologic findings. Methods: Paraffin-embedded sections from 39 superficial (T1G1-G3) and 23 invasive (T2-T4a G3 NOMO) primary transitional cell carcinomas (TCC) in the bladder were investigated immunohistochemically for p53, bcl-2 and PCNA. The median follow-up was 37 months; 24 had recurrences. The proliferation index (PI) was expressed as a percentage of the PCNA-positive cells in the tumor cells. Apoptosis was detected by terminal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and the apoptotic index (AI) was expressed as a percentage of the TUNEL-positive turnor cells. Results: P53 mutation was identified in 50 patients (80.6%). The mutation was most common in tumors of grade 3 (91.3%) as compared to grade 2 (78.5%) and grade 1 (72.7%, P<0.05). Stage pT2 tumors had a higher frequency of p53 mutation (95.7%) as compared to pTa-1 tumors (74.3%, P<0.01). Only 14 tumors (22.5%) expressed bcl-2; grade 3 tumors expressed bcl-2 significantly more frequently (P<0.05); there was no correlation between bcl-2 and tumor stage. There was no interrelation between p53 mutation and bcl-2 expression (P>0.05). The PI ranged from 17.2% to 41.8% (median 22.4%) and the AI from 1.9% to 3.5% (median 2.9%) in bladder cancer. Statistical analyses revealed a close associations between PI, AI and tumor grade and stage of bladder cancer. Conclusion: P53 mutation correlates with invasion. P53 and PCNA overexpression may offer valuable additional prognostic information in bladder tumors. With the progression of the tumor grade, cell proliferation may be accompanied by frequent apoptosis in bladder cancer, but the PI increased much more than the AI.
机译：目的：将膀胱癌患者p53突变的频率，bcl-2表达与增殖状态（增殖细胞核抗原，PCNA）与细胞增殖，凋亡及其临床病理结果联系起来。方法：对39例膀胱浅表性（T1G1-G3）和23例浸润性（T2-T4a G3 NOMO）原发性移行细胞癌（TCC）的石蜡包埋切片进行了免疫组织化学分析，研究了p53，bcl-2和PCNA。中位随访时间为37个月。 24次复发。增殖指数（PI）表示为肿瘤细胞中PCNA阳性细胞的百分比。通过末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL）检测凋亡，并且凋亡指数（AI）表示为TUNEL阳性转向细胞的百分比。结果：50例患者中检出P53突变（80.6％）。与2级（78.5％）和1级（72.7％，P <0.05）相比，突变在3级（91.3％）的肿瘤中最常见。与pTa-1肿瘤（74.3％，P <0.01）相比，pT2期肿瘤具有更高的p53突变频率（95.7％）。仅14个肿瘤（22.5％）表达bcl-2； 3级肿瘤表达bcl-2的频率更高（P <0.05）； bcl-2与肿瘤分期无相关性。 p53突变与bcl-2表达无相关性（P> 0.05）。在膀胱癌中，PI的范围为17.2％至41.8％（中位数为22.4％），AI的范围为1.9％至3.5％（中位数为2.9％）。统计分析显示PI，AI与膀胱癌的肿瘤分级和分期之间密切相关。结论：P53突变与侵袭有关。 P53和PCNA的过度表达可能为膀胱肿瘤提供有价值的其他预后信息。随着肿瘤等级的发展，膀胱癌中细胞增殖可能伴随着频繁的细胞凋亡，但是PI的增加远超过AI。

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