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首页> 外文期刊>Chinese Journal of Chemistry >Study on the Interaction between Cephalosporin Drugs and Papain by Spectroscopic Method: Equivalence of Fluorescence Quenching and Enhancement Equation
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Study on the Interaction between Cephalosporin Drugs and Papain by Spectroscopic Method: Equivalence of Fluorescence Quenching and Enhancement Equation

机译：光谱法研究头孢菌素类药物与木瓜蛋白酶的相互作用：荧光猝灭和增强方程的当量

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摘要

The interaction between two 4th generation of new cephalosporin drugs and papain was studied through spectroscopy method at different temperatures. Their dissociation constants were calculated by using theoretical formula of fluorescence quenching and fluorescence enhancement, respectively, and their corresponding thermodynamic functions, dipole-dipole non-radiative energy-transfer-efficiency and the action distances of acceptor-substrate etc. were calculated further. Analysis reveals that, the quenching on papain by cefpirome and cefepime is basically due to the dipole-dipole non-radiation energy-transfer and certain dynamic collision between donor and acceptor, the interaction between drug and papain is mainly hydrophobic which may provide useful information for pharmacological and metabolic study on papain. In this paper fluorescence quenching is regarded as negative fluorescence enhancement, and two kinds of theoretical formula of fluorescence quenching and fluorescence enhancement were used to calculate the experimental data of fluorescence quenching of a same batch, and very close results were obtained but with certain difference. The results not only display the equivalence of the two theoretical equations when treating acceptor-substrate action, but also show that the thermodynamic data based on the theoretical formula of fluorescence enhancement (4), which is deduced by us, are more reasonable. So we suggest that even in the process of studying the fluorescence quenching between receptor-substrate, using the theoretical formula of fluorescence enhancement (4) can get more reliable results.
机译：通过分光光度法研究了两种第四代头孢菌素新药与木瓜蛋白酶之间的相互作用。分别使用荧光猝灭和荧光增强的理论公式计算它们的解离常数，并进一步计算它们各自的热力学函数，偶极-偶极非辐射能量转移效率和受体-基质的作用距离等。分析表明，头孢吡肟和头孢吡肟对木瓜蛋白酶的猝灭主要是由于偶极-偶极非辐射能量转移和供体与受体之间一定的动态碰撞，药物与木瓜蛋白酶之间的相互作用主要是疏水性的，可能为木瓜蛋白酶的药理代谢研究。本文将荧光猝灭视为负荧光增强，并用荧光猝灭和荧光增强两种理论公式来计算同一批次的荧光猝灭实验数据，获得了非常接近的结果，但有一定的区别。结果不仅显示了当处理受体-底物作用时两个理论方程的等价性，而且表明我们推导的基于荧光增强理论公式（4）的热力学数据更为合理。因此我们建议即使在研究受体-底物之间的荧光猝灭的过程中，使用荧光增强的理论公式（4）也可以获得更可靠的结果。

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