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Localization of NOS-like protein in guard cells of Vicia faba L. and its possible function

机译：蚕豆保卫细胞中NOS样蛋白的定位及其可能的功能

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摘要

Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of nitric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS inhibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO generation from NOS pathway is the main pathway in the stress and JA responses. The pharmacological experiment showed that increasing the Ca~(2+) at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca~(2+)-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses.
机译：利用免疫荧光和免疫金电子显微镜技术，确定蚕豆保卫细胞中一氧化氮合酶（NOS）样蛋白的定位。NOS主要定位在细胞核，细胞质，叶绿体，线粒体和细胞壁中保卫细胞。灼热和外源JA可以提高叶片和表皮中一氧化氮（NO）的水平并增加NOS活性，且NOS活性的变化模式与NO的变化一致。 NOS抑制剂L-NAME抑制JA诱导的NO生成。根据结果​​，我们推测NOS途径的NO生成是应激和JA反应的主要途径。药理实验表明，以适当的浓度增加Ca〜（2+）可以促进叶片NOS活性和NO水平，表明NOS活性以及NO的分布是Ca〜（2+）依赖性的。 NOS和NO可能参与气孔运动的调节，因此在植物胁迫反应中起重要作用。

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