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首页> 外文期刊>Chinese science bulletin >Potential existence of two independent centrosome-targeting domains in PP4
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Potential existence of two independent centrosome-targeting domains in PP4

机译：PP4中可能存在两个独立的靶向中心体的域

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摘要

Protein phosphatase 4 (PP4) is an important member in the PPP family of protein Ser/Thr phosphatases. It has been proven to regulate a variety of cellular processes such as centrosome maturation, micro-tubule nucleation, splicesome assembly, and JNK pathway activation. Compared to the crystallized and structurally well defined phosphatase PP1 and PP2B, little is known about the structure of PP4. Besides the conserved motifs characteristic of the PPP family, no information is available on the other domains of PP4. PP4 is reported to localize to the centrosome in many species such as Drosophila, Caenor-habditis elegans and mammalian cells, which suggests a conserved role of PP4 in the regulation of centrosome function. Unlike several other centrosomal proteins, no sequence has been identified for PP4 that can target it to specific centrosomal localization. In this study, we used a combination of PCR mutagenesis and transient expression of GFP-tagged proteins in mammalian cells, and identified two PP4 centrosome-targeting domains of 68—136 and 134—220 aa. These two domains may be associated for appropriate localization to the centrosome. The findings are useful for further elucidating the function of the domains and other structural characteristics of PP4.
机译：蛋白质磷酸酶4（PP4）是PPP Ser / Thr磷酸酶家族中的重要成员。已经证明它可以调节多种细胞过程，例如中心体成熟，微管成核，剪接体组装和JNK途径激活。与结晶且在结构上明确定义的磷酸酶PP1和PP2B相比，对PP4的结构知之甚少。除了PPP家族的保守基序特征外，没有关于PP4其他域的信息。据报道，PP4在许多物种中（例如果蝇，秀丽隐杆线虫和哺乳动物细胞）都定位于中心体，这表明PP4在中心体功能调节中的保守作用。与其他几种中心体蛋白不同，尚未鉴定出可将PP4靶向特定中心体定位的序列。在这项研究中，我们结合了PCR诱变和GFP标记蛋白在哺乳动物细胞中瞬时表达的组合，并鉴定了两个PP4中心体靶向结构域68-136和134-220aa。可以将这两个结构域相关联以适当定位于中心体。该发现对于进一步阐明PP4的域功能和其他结构特征是有用的。

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