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Exogenous cAMP upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021

机译:外源性cAMP上调苜蓿中华根瘤菌1021中glnII和glnK-amtB基因的表达

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The existence of multiple adenylate cy-clase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021, In this study, as a pioneer step of understanding cAMP roles, mi-croarray analysis on S. meliloti was carried out for the function of exogenous cAMP. To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed in S. meliloti that the expression of either glnII or glnk promoter-lacZtranslational fusion is higher in the presence of exogenous cAMP. Therefore, for the first time, we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregula-tion of glnII and glnK by cAMP is discussed.
机译:多个腺苷酸环化酶编码基因的存在暗示了cAMP在Melinotizobium meliloti 1021中的重要性。在这项研究中,作为了解cAMP作用的开创性步骤,对S.meliloti进行了微阵列分析以分析外源cAMP的功能。 。令我们惊讶的是,该结果表明,在苜蓿链球菌中存在外源性cAMP时,glnII和glnK基因的转录明显上移。该现象在苜蓿链球菌中进一步得到证实,在存在外源性cAMP的情况下,glnII或glkk启动子-lacZ翻译融合体的表达更高。因此,我们首次从苜蓿链球菌中鉴定了其表达被cAMP激活的基因。讨论了cAMP上调glnII和glnK的潜在生理作用。

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