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首页> 外文期刊>Chinese science bulletin >Hydrogen production by draTGB hupL double mutant of Rhodospirillum rubrum under different light conditions
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Hydrogen production by draTGB hupL double mutant of Rhodospirillum rubrum under different light conditions

机译：在不同光照条件下由红景天螺旋藻的draTGB hupL双突变体产氢

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摘要

To increase H_2 yield of Rhodospirillum rubrum in two-stage hydrogen production process, two deletion mutants were constructed. One is single mutant designated R. rubrum UR801 that deleted hupL gene encoding the large subunit of uptake hy-drogenase, and the other is a double mutant designated R. rubrum UR805 lacked both draTGB encoding regulators for the activity of nitrogenase and hupL. Comparing H_2 yields of two mutants with R. rubrum UR2 (wild type) and UR472 (AdraTGB) under different light conditions, the results showed that the H_2 yield of R. rubrum UR801 under continuous light is the highest (5700 ml of H_2 per liter culture), and it is 1.56, 2.24 and 2.32-fold that of R. rubrum UR2, UR472 and UR805, respectively. However, the total H_2 yield of R. rubrum UR805 in two-stage hydrogen production process is the highest (4303 mL/L), and it is 1.35, 1.21 and 1.04-fold that of R. rubrum UR2, UR801 and UR472, respectively. Thus, R. rubrum UR805 might be a valuable strain to produce a large amount of hydrogen in two-stage hydrogen production process.
机译：为了在两步制氢过程中提高红球螺旋藻的H_2产量，构建了两个缺失突变体。一个是命名为R. rubrum UR801的单突变体，它删除了编码摄取水合氢酶的大亚基的hupL基因，另一个是命名为R. rubrum UR805的双突变体，它既缺乏对dratGB编码的固氮酶和hupL活性的调控因子。在不同光照条件下，将两个突变体与R. rubrum UR2（野生型）和UR472（AdraTGB）的H_2产量进行比较，结果表明，连续光照下R. rubrum UR801的H_2产量最高（每升5700 ml H_2）文化），分别是红景天UR2，UR472和UR805的1.56倍，2.24倍和2.32倍。然而，在两级制氢过程中，R。rubrum UR805的总H_2产量最高（4303 mL / L），分别是R. rubrum UR2，UR801和UR472的1.35、1.21和1.04倍。 。因此，R。rubrum UR805可能是在两步制氢过程中产生大量氢气的有价值的菌株。

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