Sequence analysis on biological active peptides using electro-spray ionization-Fourier transform ion cyclotron resonance mass spectrometer

CHEN Hui; XU Jiaxi; XIN Bin

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Sequence analysis on biological active peptides using electro-spray ionization-Fourier transform ion cyclotron resonance mass spectrometer

机译：电喷雾电离-傅立叶变换离子回旋共振质谱仪对生物活性肽的序列分析

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This paper describes experience with the sequencing of synthetic segment peptides of gp41 which, are used in trimeric peptides as potential peptide-based vaccines and as inhibitors to mimic the fusion state of gp41 in HIV infection using ESI/CID-FT-ICR MS. AH b and y" series ions are found in the CID spectra and the mass difference between the calculated and observed results is very slight. FT-ICR MS again shows its power in peptide sequencing, successfully helping us obtain the sequence of an unknown

机译：本文介绍了对gp41合成节段肽进行测序的经验，该合成节段肽用于三聚体肽中作为潜在的基于肽的疫苗，并用作抑制剂，使用ESI / CID-FT-ICR MS模拟gp41在HIV感染中的融合状态。在CID光谱中发现了AH b和y“系列离子，计算结果和观察到的结果之间的质量差异非常小。FT-ICR MS再次显示了其在肽测序中的功能，成功地帮助我们获得了未知序列

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来源
《Chinese science bulletin》 |2005年第9期|p.853-857|共5页
作者
CHEN Hui; XU Jiaxi; XIN Bin;
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作者单位

Department of Chemistry, Peking University, Beijing 100871, China;

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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
peptide; ESI; FT-ICR MS; SORI-CID;

机译：肽;ESI;FT-ICR MS;SORI-CID;

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专利

1. Sequence analysis on biological active peptides using electro-spray ionization-Fourier transform ion cyclotron resonance mass spectrometer [J] . CHEN Hui, XU Jiaxi, XIN Bin Chinese science bulletin . 2005,第9期

机译：电喷雾电离-傅立叶变换离子回旋共振质谱仪对生物活性肽的序列分析
2. Sequence and modified group analysis on C-terminal modified analogs of endomorphin-2 using electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometer [J] . ZHU ChuanJun, HU ChangFeng, SHAO Xuan Science in China, Series B. Chemistry . 2008,第1期

机译：使用电喷雾电离-傅立叶变换离子回旋共振质谱仪对内啡肽2的C端修饰类似物进行序列分析和修饰基团分析
3. Integration of electrokinetic-based multidimensional separation/concentration platform with electrospray ionization-Fourier transform ion cyclotron resonance-mass spectrometry for proteome analysis of Shewanella oneidensis [J] . Mohan D., Pasa-Tolic L., Masselon CD., Analytical chemistry . 2003,第17期

机译：基于电动学的多维分离/浓缩平台与电喷雾电离-傅立叶变换离子回旋共振质谱法的集成，可用于拟南芥的蛋白质组分析
4. Urinary metabolomic analysis by ultra-high performance liquid chromatography/ Fourier transform ion cyclotron resonance mass spectrometer and ion trap mass spectrometer [C] . Yi-Ting Kao, Yi-Ting Chen, Chien-Lun Chen, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：超高效液相色谱/傅里叶变换离子回旋谐振质谱仪和离子阱质谱仪尿代谢分析
5. Online combination of multidimensional electrokinetic separations with electrospray ionization-Fourier transform ion cyclotron resonance-mass spectrometry for proteome analysis. [D] . Mohan, Deepa. 2003

机译：多维电动分离与电喷雾电离-傅立叶变换离子回旋共振质谱的在线结合，用于蛋白质组分析。
6. Evaluation of a Novel Approach for Peptide Sequencing: Laser-induced Acoustic Desorption Combined with ... formula ... Chemical Ionization and Collision-activated Dissociation in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer [O] . Jayalakshmi Somuramasami, Hilkka I. Kenttämaa -1

机译：肽测序的新方法的评估：激光诱导的声解吸结合...公式...傅里叶变换离子回旋共振质谱仪中的化学电离和碰撞活化解离
7. A high performance low magnetic field internal electrospray ionization-fourier transform ion cyclotron resonance mass spectrometer [O] . Wu Qinyuan, Anderson Gordon A., Udseth Harold R., 1996

机译：高性能低磁场内部电喷雾电离-傅立叶变换离子回旋共振质谱仪

Sequence analysis on biological active peptides using electro-spray ionization-Fourier transform ion cyclotron resonance mass spectrometer

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