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首页> 外文期刊>Chinese science bulletin >Apoptosis of Spodoptera litura larval hemocytes induced by heavy metal zinc
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Apoptosis of Spodoptera litura larval hemocytes induced by heavy metal zinc

机译:重金属锌诱导斜纹夜蛾幼虫血细胞凋亡

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摘要

By adding different amount of zinc into the artificial medium of the insect larvae, the zinc-induced apoptosis of the larvae haemocytes of the herbivorous insect Spodoptera litura Fabricius was investigated with flow cy-tometer. The results showed that the increase of zinc dose in the artificial feed led to the accumulations of zinc in the larval hemolymph and fat body, and more zinc was accumulated in fat body than in hemolymph. The apoptosis of hemocytes was significantly induced at high zinc concentration (1000 mg·kg~(-1)) in the insect diet, and the apoptosis rate was 63.63%, which was remarkably higher than that at control and lower concentrations (50—500 mg·kg~(-1)). This suggests that the high dose of zinc in the artificial diet of S. litura larvae could induce the apoptosis of the larval hemocytes of S. litura.
机译:通过向昆虫幼虫的人工培养基中添加不同量的锌,用流式细胞仪研究了锌诱导的食草昆虫斜纹夜蛾幼虫血细胞的凋亡。结果表明,人工饲料中锌剂量的增加导致幼虫血淋巴和脂肪体内锌的积累,而脂肪体内的锌比血淋巴中的锌更多。昆虫饮食中高锌浓度(1000 mg·kg〜(-1))能明显诱导血细胞凋亡,其凋亡率达63.63%,明显高于对照组和较低浓度(50-500)时的血细胞凋亡率。 mg·kg〜(-1))。这表明人造饮食的斜纹夜蛾幼虫中高剂量的锌可诱导斜纹夜蛾幼虫血细胞的凋亡。

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