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首页> 外文期刊>Chinese science bulletin >Effect of GbKTN1 from Gossypium barbadense on cell elongation of fission yeast (Schizosaccharomyces pombe)
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Effect of GbKTN1 from Gossypium barbadense on cell elongation of fission yeast (Schizosaccharomyces pombe)

机译：巴贝糖棉中GbKTN1对裂殖酵母（Schizosaccharomyces pombe）细胞伸长的影响

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摘要

The GbKTN1 gene was isolated from 10 DPA fiber cells of Gossypium barbadense using 5'RACE/3 'RACE. Full-length cDNA of this gene is 2006 bp, including a 113 bp of 5'untranslated region, a 1563 bp of an open reading frame (ORF), and a 327 bp of 3'untranslated region (excluding the stop codon TAA). The ORF of GbKTN1 encodes a 521-amino acid protein with a predicted size of 55 kD. Near C-terminal of the deduced protein there is a putative ATP binding site between amino acid residues from 233 to 414. Southern blot analysis indicated that the GbKTN1 was a single copy gene in G. barbadense. Combining semi-quantitative RT-PCR with Southern blot hybridization revealed that GbKTN1 expressed in all the organs detected such as roots, stems, leaves and fibers. However, the mRNA of GbKTN1 was the most abundant in fiber cells, while it was the lowest in leaves. The GbKTN1 cDNA was transformed into S. pombe to verify its function on cell elongation. Results showed that most yeast cells over expressing GbKTN1 gene were elongated dramatically with an average length increase of 2.18 times than that of the non-induced cells. Even the morphology of some yeast cells appeared irregularly. To the best of our knowledge this is the first evidence that KTN1 is correlated with cell elongation in vivo.
机译：使用5'RACE / 3'RACE从10个古巴棉DPA纤维细胞中分离出GbKTN1基因。该基因的全长cDNA为2006 bp，包括113 bp的5'非翻译区，1563 bp的开放阅读框（ORF）和327 bp的3'非翻译区（不包括终止密码子TAA）。 GbKTN1的ORF编码一个521个氨基酸的蛋白质，预测大小为55 kD。在推测的蛋白质的C末端附近，从233到414的氨基酸残基之间存在一个假定的ATP结合位点。Southern印迹分析表明GbKTN1是巴巴德氏酵母中的单拷贝基因。将半定量RT-PCR与Southern印迹杂交相结合，发现在检测到的所有器官（如根，茎，叶和纤维）中都表达了GbKTN1。然而，GbKTN1的mRNA在纤维细胞中含量最高，而在叶片中最低。 GbKTN1 cDNA被转化到粟酒裂殖酵母中，以验证其对细胞伸长的作用。结果表明，大多数过表达GbKTN1基因的酵母细胞被显着延长，其平均长度比未诱导的细胞增加了2.18倍。甚至一些酵母细胞的形态也不规则地出现。据我们所知，这是第一个证据表明KTN1与体内细胞伸长相关。

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