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首页> 外文期刊>Chinese science bulletin >Cloning and roles of goldfish maternal factor beta-Catenin cDNA in embryonic development
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Cloning and roles of goldfish maternal factor beta-Catenin cDNA in embryonic development

机译：金鱼母亲因子β-CatenincDNA的克隆及其在胚胎发育中的作用

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摘要

Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. beta-Catenin, an important maternal factor in early embryo-genesis, has been analyzed in its expression pattern and functions in this paper. We have cloned goldfish beta-Catenin cDNA gene and compared it with zebrafish beta -Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93 percent (2227/2384 bp) and 98.5 percent (768/780 aa) respectively. The expression pattern of beta-Catenin by in situ hybridization and the roles of beta-Catenin on embryonic development by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that beta-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of beta-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposterior axes, and leads embryos to die before larva get hatched.
机译：核与细胞质之间的相互作用已经集中在动物胚胎发育领域，在该领域中积极地需要研究母体因子。 β-连环蛋白是早期胚胎发生中的重要母体因子，已对其表达模式和功能进行了分析。我们已经克隆了金鱼β-CatenincDNA基因，并将其与斑马鱼β-CatenincDNA进行了比较。在cDNA和它们之间的氨基酸序列中发现高度同源性，分别为93％（2227/2384 bp）和98.5％（768/780 aa）。在金鱼胚胎发育的整个过程中，研究了原位杂交产生的β-Catenin的表达模式以及β-Catenin在胚胎发育中的作用，方法是共注入反义RNA和报告基因EGFP。结果表明，β-连环素呈动态分布，受精后主要位于体轴，背​​组织，头尾结构。 β-Catenin活性的丧失会严重破坏背侧组织和前后轴的胚胎，并导致胚胎在幼虫孵化之前死亡。

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