A novel method of DNA shuffling without PCR process

WANG Qiang; LIU Oiuyun; LI Gang

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A novel method of DNA shuffling without PCR process

机译：无需PCR即可进行DNA改组的新方法

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摘要

Most DNA shuffling methods currently used require PCR process. A novel method of DNA shuffling without PCR process is described, taking advantage of the feature of some restriction enzymes whose recognition sites differ from their cleavage sites, thus giving rise to different cohesive ends. These cohesive ends can be rejoined at their native sites from different parental sequences, generating new sequences with various combinations of mutations.

机译：当前使用的大多数DNA改组方法需要PCR过程。描述了一种无需PCR过程的DNA重排的新方法，该方法利用了一些限制性酶的特征，这些酶的识别位点不同于其裂解位点，从而产生了不同的内聚末端。这些有粘性的末端可以从不同的亲本序列在其天然位点重新连接，从而产生具有各种突变组合的新序列。

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来源
《Chinese science bulletin》 |2004年第7期|p.689-691|共3页
作者
WANG Qiang; LIU Oiuyun; LI Gang;
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作者单位

Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Zhongshan University, Guangzhou 510275, China;

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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
DNA shuffling; restriction enzyme; tissue plasminogen activator;

机译：DNA改组;限制性内切酶;组织纤溶酶原激活物;

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1. A novel method of DNA shuffling without PCR process [J] . WANG Qiang, LIU Oiuyun, LI Gang Chinese science bulletin . 2004,第7期

机译：无需PCR即可进行DNA改组的新方法
2. Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real- time PCR assay [J] . Food Chemistry . 2020,第Mara30期

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3. Detection without sample processing of yoghurt starters and DNA in milk products by PCR [J] . S. Lick, K. Schneede, A. Wittke, Kieler Milchwirtschaftliche Forschungsberichte . 1999,第1期

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A novel method of DNA shuffling without PCR process

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