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首页> 外文期刊>Chinese science bulletin >Obtaining the transgenic poplars with low lignin content through down-regulation of 4CL
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Obtaining the transgenic poplars with low lignin content through down-regulation of 4CL

机译：通过下调4CL获得低木质素含量的转基因杨树

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摘要

The antisense 4CL (4-coumarate: CoA ligase) gene was transformed into triploid Chinese white poplar (Populus tomentosa) mediated by Agrobacterium tumefaciens. PCR and Southern blot analysis indicated that antisense 4CL gene had been integrated into the genome of the transgenic Chinese white poplars. The antisense gene had also been expressed, which was indicated by RT-PCR and Western analysis. Klason lignin content assay showed that repression of 4CL expression could result in remarkable +reduction of lignin content in transgenic poplars, with most reduction of 41.73 percent compared with that of wild type in this paper. But there is no significant difference in holocellulose content between trans-genic and wild poplars. We considered that 4CL might not be the metabolism control point between lignin and carbohy-drate biosynthesis. The stems of transgenic poplars displayed red-brown color with different levels after the bark was peeled, while those of untransformed plants were white. No visible differences in growth and development were observed between transgenic and wild plants. Wiesner reaction analysis of the transgenic plant stems with reduced lignin content exhibited red color, while that of untransformed plant was typically purple-red.
机译：将反义4CL（4-香豆酸酯：CoA连接酶）基因转化为根癌农杆菌介导的三倍体中国白杨（Populus tomentosa）。 PCR和Southern blot分析表明，反义4CL基因已经整合到转基因中国白杨的基因组中。还已经表达了反义基因，其通过RT-PCR和Western分析表明。 Klason木质素含量测定表明，抑制4CL表达可能导致转基因杨树中木质素含量显着+降低，与野生型相比，降低幅度最大为41.73％。但是，在转基因和野生杨之间全纤维素含量没有显着差异。我们认为4CL可能不是木质素和碳酸盐生物合成之间的代谢控制点。剥皮后，转基因杨树的茎显示出不同程度的红棕色，而未转化的植物的茎则显示为白色。在转基因植物和野生植物之间未观察到生长和发育的明显差异。木质素含量降低的转基因植物茎的Wiesner反应分析显示为红色，而未转化植物的Wiesner反应分析通常为紫红色。

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