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首页> 外文期刊>Chinese science bulletin >Cytoplasmic male sterility of tuber mustard is associated with the alternative spliced mitochondrial T gene transcripts
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Cytoplasmic male sterility of tuber mustard is associated with the alternative spliced mitochondrial T gene transcripts

机译：块茎芥菜的细胞质雄性不育与选择性剪接的线粒体T基因转录物有关

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摘要

Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondria! cDNA of tuber mustard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type II intron. The expressions of the two transcripts were analyzed by reverse transcription PCR (RT-PCR). The results showed that, at seedling stage, the expression of T gene was mainly in the form of T1170 hot decreased with the development gradually, while the expression abundance of another transcript, T1243, increased gradually. The T1243 was prevalent at the profuse floweringtThe expression pattern was confirmed by Northern blot analysis. These results suggested the alternative spliced mitochondria! T gene transcripts were related to CMS of tuber mustard.
机译：从线粒体获得了T基因的两个转录物T1170和T1243！芥菜CMS系cDNA。 T1243是具有未剪接的内含子的转录物，其具有II型内含子的基本特征。通过逆转录PCR（RT-PCR）分析了两个转录物的表达。结果表明，在苗期，T基因的表达主要以T1170 hot的形式表达，并随其发育逐渐降低，而另一种转录本T1243的表达丰度则逐渐升高。 T1243在大量开花时盛行。通过Northern印迹分析证实了表达模式。这些结果表明替代剪接的线粒体！ T基因转录本与块茎芥菜的CMS有关。

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