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首页> 外文期刊>Chinese science bulletin >Molecular characterization of Tomato yellow leaf curl China virus and its satellite DNA isolated from tobacco
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Molecular characterization of Tomato yellow leaf curl China virus and its satellite DNA isolated from tobacco

机译：番茄黄叶卷曲中国病毒的分子特征及其从烟草中分离得到的卫星DNA

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摘要

Virus isolates, Y8, Y36 and Y38, were obtained from tobacco plants showing leaf curl symptoms in Honghe, Yunnan Province. In reactions with 14 monoclonal antibodies raised against Begomovirus particles, Y8, Y36 and Y38 had similar antigenic reaction in TAS-ELISA as Tomato yellow leaf curl China virus (TYLCCNV). The complete DNA-A nucleotide sequences of Y8, Y36 and Y38 were determined and they contain 2727, 2730 and 2730 nucleotides, respectively. Each of the DNA-A sequences has a typical Begomovirus genome organization encoding 6 ORFs with 2 ORFs [AV1(CP) and AV2] in virion-sense DNA and 4 ORFs (AC1 to AC4) in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs show that Y8, Y36 and Y38 are isolates of TYL CCNV. Satellite DNA molecules (DNAβ) were found to be associated with Y8, Y36 and Y38, which consist of 1338, 1339 and 1338 nucleotides, respectively. Comparisons show that these DNAβ molecules share 98%-99% sequence identities on nucleotide level and have a common ORF (designated C1) encoding 126 amino acids on the complementary strand.
机译：Y8，Y36和Y38病毒分离株是从云南省红河市出现叶卷曲症状的烟草植株中获得的。在与针对乙型肝炎病毒颗粒的14种单克隆抗体反应中，Y8，Y36和Y38在TAS-ELISA中的抗原反应与番茄黄叶卷曲中国病毒（TYLCCNV）相似。确定了Y8，Y36和Y38的完整DNA-A核苷酸序列，它们分别包含2727、2730和2730个核苷酸。每个DNA-A序列都有一个典型的牛瘟病毒基因组结构，在病毒体有义DNA中编码6个ORF，其中有2个ORF [AV1（CP）和AV2]，在互补义DNA中编码4个ORF（AC1至AC4）。与单个ORF的总DNA-A，基因间区域和推导的氨基酸序列的比较表明，Y8，Y36和Y38是TYL CCNV的分离株。发现卫星DNA分子（DNAβ）与Y8，Y36和Y38相关，它们分别由1338、1339和1338个核苷酸组成。比较显示，这些DNAβ分子在核苷酸水平上具有98％-99％的序列同一性，并在互补链上具有编码126个氨基酸的共同ORF（称为C1）。

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