Real time monitoring of nucleic acids ligation based on molecular beacon

TANG Zhiwen; WANG Kemin; TAN Weihong; LI Jun; LIU Lingfeng; GUO Qiuping; MENG Xiangxian; HUANG Shasheng; LI Du; LUO Weifang

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Real time monitoring of nucleic acids ligation based on molecular beacon

机译：基于分子信标的核酸连接实时监控

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摘要

A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluorescence signal quickly and quantitatively. This method provides effective and original approach to researching the dynamic ligation process and the interactions between nucleic acids and ligase. An analytical method for T4 DNA ligase based on this way has been built up with a linear detection range from 2.3 x 10~(-4) U/mL to 0.23 U/mL. It is rapid and sensitive to detect 2.8 x 10~(-5) U T4 DNA ligase in 10 min.

机译：已经开发了监测核酸连接过程的新方法。此处使用分子信标将连接信息快速定量地转换为荧光信号。该方法为研究动态连接过程以及核酸与连接酶之间的相互作用提供了有效而原始的方法。建立了基于这种方法的T4 DNA连接酶分析方法，其线性检测范围为2.3 x 10〜（-4）U / mL至0.23 U / mL。在10分钟内检测到2.8 x 10〜（-5）U T4 DNA连接酶快速而灵敏。

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来源
《Chinese science bulletin》 |2003年第12期|p.1215-1218|共4页
作者
TANG Zhiwen; WANG Kemin; TAN Weihong; LI Jun; LIU Lingfeng; GUO Qiuping; MENG Xiangxian; HUANG Shasheng; LI Du; LUO Weifang;
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作者单位

State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;

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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
nucleic acids ligation; T4 DNA ligase; real time; assay; molecular beacon;

机译：核酸连接;T4 DNA连接酶;实时;测定;分子信标;

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1. Real-Time Monitoring of Nucleic Acid Dephosphorylation by Using Molecular Beacons [J] . Changbei Ma, Zhiwen Tang, Kemin Wang Chembiochem: A European journal of chemical biology . 2007,第13期

机译：使用分子信标实时监测核酸脱磷酸
2. Monitoring HCV RNA viral load by locked nucleic acid molecular beacons real time PCR. [J] . Morandi L, Ferrari D, Lombardo C, Journal of Virological Methods . 2007,第1a2期

机译：通过锁定核酸分子信标实时PCR监测HCV RNA病毒载量。
3. Development of a Quantitative Real-Time Nucleic Acid Sequence-Based Amplification Assay with an Internal Control Using Molecular Beacon Probes for Selective and Sensitive Detection of Human Rhinovirus Serotypes [J] . Francesca Sidoti, Massimiliano Bergallo, Maria Elena Terlizzi, Molecular Biotechnology . 2012,第3期

机译：使用分子信标探针对人类鼻病毒血清型进行选择性和灵敏检测的内部对照定量实时核酸序列扩增试验的开发。
4. Locked Nucleic Acid Molecular Beacons for Intracellular mRNA Monitoring [C] . Lin Wang, Chaoyong James Yang, Colin D. Medley, Nanobiophotonics and Biomedical Applications III; Progress in Biomedical Optics and Imaging; vol.7, no.18 . 2006

机译：锁定核酸分子信标用于细胞内mRNA监测
5. Development and optimization of dual FRET-molecular beacons for the detection and visualization of single-stranded nucleic acid targets. [D] . Tsourkas, Andrew. 2002

机译：开发和优化用于检测和可视化单链核酸靶标的双FRET分子信标。
6. Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons [O] . Zhiwen Tang, Kemin Wang, Weihong Tan, 2003

机译：使用分子信标实时监测均质溶液中的核酸连接
7. Rapid Real-Time Nucleic Acid Sequence-Based Amplification-Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections ▿ [O] . Zhao, Yanan, Park, Steven, Kreiswirth, Barry N., 2009

机译：基于实时核酸序列的快速扩增-分子信标平台，可检测真菌和细菌的血流感染
8. NanoCluster Beacon - A New Molecular Probe for Homogeneous Detection of Nucleic Acid Targets. [R] . Yeh, H., Sharma, J., Han, J. J., 2011

机译：NanoCluster Beacon - 核酸靶均相检测的新分子探针。

Real time monitoring of nucleic acids ligation based on molecular beacon

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