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首页> 外文期刊>Chinese science bulletin >Over-expression of an Arabi- dopsis δ-OAT gene enhances salt and drought tolerance in transgenic rice
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Over-expression of an Arabi- dopsis δ-OAT gene enhances salt and drought tolerance in transgenic rice

机译：拟南芥δ-OAT基因的过表达增强了转基因水稻的耐盐和耐旱性

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摘要

δ-OAT, ornithine-δ-aminotransferase, is the key enzyme involved in proline biosynthesis. In this study the Arabidopsis δ-OAT gene was transferred into rice (Oryza sativa L. ssp japonica cv. Zhongzuo 321), whose successful integration was demonstrated by PCR and Southern blot analysis. The over-expression of the gene in transgenic rice was also confirmed. Biochemical analysis showed that, under salt or drought stress conditions, proline Contents in the leaves and roots in transgenic rice plants were 5- to 15-fold of those in non-transgenic controls. Under stress conditions, germinating rate of transgenic lines is higher than that of controls. Although the growth of rice plants tested were more and more retarded with the increasing of NaCl concentration, the transgenic plants grow faster compared to the controls under the same stress condition. Meanwhile, the resistance to KCl and MgSO_4 stresses was also found enhanced in transgenic rice. Furthermore, the over-expression of δ-OAT also improved the yield of transgenic plants under stress conditions. The average yield per plant of transgenic lines in-creases about 12% — 41% more than that of control lines under 0.1 mol/L NaCl stress. These data indicated that the over-expression of δ-OAT, with the accumulation of proline, resulted in the enhancement of salt and drought tolerance and an increase of rice yield, which is of significance in agri-culture.
机译：δ-OAT，鸟氨酸-δ-氨基转移酶是脯氨酸生物合成中涉及的关键酶。在这项研究中，拟南芥δ-OAT基因被转移到水稻（Oryza sativa L. ssp japonica cv。Zhongzuo 321）中，通过PCR和Southern blot分析证明其成功整合。还证实了该基因在转基因水稻中的过表达。生化分析表明，在盐或干旱胁迫条件下，转基因水稻植株的叶和根中脯氨酸含量是非转基因对照植物的5至15倍。在胁迫条件下，转基因品系的发芽率高于对照。尽管随着NaCl浓度的增加，受试水稻植株的生长受到越来越多的阻碍，但在相同胁迫条件下，转基因植株的生长速度要比对照植株快。同时，还发现转基因水稻对KCl和MgSO_4胁迫的抗性增强。此外，δ-OAT的过表达也提高了逆境条件下转基因植物的产量。在0.1 mol / L NaCl胁迫下，转基因品系的单株平均产量比对照品系提高约12％– 41％。这些数据表明，δ-OAT的过表达和脯氨酸的积累导致盐分和耐旱性的提高以及水稻产量的提高，这在农业中具有重要意义。

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