Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

Liu Zhiyi; Xiang Jianhai; Zhou Guoying

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Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

机译：用基因枪将外源基因转移到中国虾（对虾）中

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Plasmids pG DNA-RZI with a GFP (green fluorescent protein) reporter gene and a ribozyme gene in - cising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different de - velopment stages were observed with a fluorescent micro - scope. The transient expression of GFP gene was high in nauplius and zoea larvae.

机译：首先，用基因枪将带有绿色荧光蛋白报道基因和核酶基因的pG DNA-RZI质粒导入对虾白斑杆状病毒（WSBV）中。用荧光显微镜观察了不同发育阶段的处理样品和对照样品。 GFP基因的瞬时表达在无节幼体和zoea幼虫中较高。

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《Chinese science bulletin》 |2001年第9期|p.766-769|共4页
作者
Liu Zhiyi; Xiang Jianhai; Zhou Guoying;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
gene gun; GFP; transgenic shrimps.;

机译：基因枪GFP;转基因虾。;

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专利

1. Sequence Variation in the Farnesoic Acid O-methyltransferase Gene and its Associations with Growth of Shrimp, Penaeus chinensis [J] . Li Zhaoxia, Wang Jinye, Zhang Yan, Journal of the World Aquaculture Society . 2015,第4期

机译：法尼乙酸O-甲基转移酶基因的序列变异及其与虾对虾的生长相关
2. Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. [J] . Zhang TianShi, Kong Jie, Wang WeiJi, Aquaculture . 2010,第1a2期

机译：用微卫星评估了中国对虾对虾的繁殖种群的遗传变异性。
3. Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China [J] . Zhang Tianshi, Kong Jie, Wang Weiji, Aquaculture . 2010,第1a2期

机译：用微卫星技术评估中国对虾对虾繁殖种群的遗传变异
4. Assesment of Genetic Variation in outbreed and inbreed Giant Tiger Shrimp (Penaeus monodon Fabricius, 1798) using ISSR marker [C] . Arifah Pumamaningrum, Niken Satuti Nur Handayani, Trijoko, International Conference on Science and Technology . 2016

机译：assr标记的遗传和巨型巨虎虾（Penaeus Monodon Fabricius，1798）的遗传变异的判断
5. Morphologic and genetic characterization of wild populations of shrimp of the genus Penaeus within the Gulf of California, Mexico: New social, political, and management dilemmas for the Mexican shrimp fishery. [D] . Aubert, Hernan. 1997

机译：墨西哥湾墨西哥对虾属对虾野生种群的形态和遗传特征：墨西哥对虾渔业的新的社会，政治和管理困境。
6. Genetic Distances between Two Cultured Penaeid Shrimp(Penaeus chinensis) Populations Determined by PCRAnalysis [O] . Jong-Man Yoon 2019

机译：两种养殖对虾的遗传距离PCR确定的中华对虾（Penaeus chinensis）种群分析
7. Enzyme polymorphism and phylogenetic relationships of the shrimps, Penaeus and Metapenaeus, from Southern China. [O] . 1992

机译：中国南方虾，对虾和新对虾的酶多态性和系统发育关系。

Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

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