Crystallization and prelimi - nary X-ray crystallographic analysis of yeast prion protein Ure2p with shortened N-terminal

Wang Liwei; Zhu Li; Zhou Junmei

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Crystallization and prelimi - nary X-ray crystallographic analysis of yeast prion protein Ure2p with shortened N-terminal

机译：N端缩短的酵母蛋白Ure2p的结晶和初步X射线晶体学分析

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An orthorhombic crystal form of a recombi- nant yeast prion protein with shortened N-terminal, 90Ure2p, has been obtained. Crystals were grown by the vapordiffu - sion technique against a mother liquor containing imidazole. Crystals belong to the primitive orthorhombic lattice with The cells parameters a = 54.5 A, b = 74.7 A, c = 131.0 A. The Crystals diffract to beyond 3.0 A resolution at a synchrotron Beamline.

机译：已经获得了具有缩短的N末端90Ure2p的重组酵母病毒蛋白的斜方晶型。晶体通过气相扩散法在含咪唑的母液中生长。晶体属于原始正交晶格，其晶胞参数a = 54.5 A，b = 74.7 A，c = 131.0A。晶体在同步加速器光束线处衍射至超过3.0 A的分辨率。

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《Chinese science bulletin》 |2001年第12期|p.1012-1014|共3页
作者
Wang Liwei; Zhu Li; Zhou Junmei;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
yeast prion; Ure2p; crystallization.;

机译：酵母pr病毒Ure2p;结晶。;

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1. Prion-lnducing Domain of Yeast Ure2p and Protease Resistance of Ure2p in Prion-Containing Cells [J] . Daniel C. Masison, Reed B. Wickner Science . 1995,第5233期

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2. Hydrogen/Deuterium Exchange Mass Spectrometric Analysis of Conformational Changes Accompanying the Assembly of the Yeast Prion Ure2p into Protein Fibrils [J] . Virginie Redeker, Frederic Halgand, Luc Bousset, Journal of Molecular Biology . 2007,第4期

机译：氢/氘交换质谱分析伴随酵母Pri病毒Ure2p组装成蛋白质原纤维的构象变化
3. An Engineered PrPsc-like Molecule from the Chimera of Mammalian Prion Protein and Yeast Ure2p Prion-inducing Domain [J] . Shao-ManYIN, Man-SunSY, PoTIEN 生物化学与生物物理学报：英文版 . 2004,第002期

机译：从哺乳动物Pri蛋白的嵌合体和酵母Ure2p on诱导域的工程化的PrPsc样分子。
4. QCM-Analyses of the Intermolecular Interaction between Prion Protein N-Terminal Domains Having Increased Number of Octapeptide Repeat [C] . Keiko Sato, Yuji Horiuchi, Ayami Matsushima Japanese peptide symposium . 2011

机译：朊病毒蛋白质N-末端域之间分子间相互作用的QCM分析，其八肽重复数量增加
5. 1. X-ray crystallographic analyses of the gene V protein from Ff bacteriophage, a single-stranded DNA-binding protein, and its Y41 mutants 2. X-ray crystallographic analyses of several anticancer drugs and their interactions with DNA. [D] . Guan, Yue. 1994

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7. Crystallization and preliminary X-ray crystallographic analysis of a 40 kDa N-terminal fragment of the yeast prion-remodeling factor Hsp104 [O] . Lee, Sukyeong, Tsai, Francis T. F. 2007

机译：酵母病毒重塑因子Hsp104的40 kDa N端片段的结晶和初步X射线晶体学分析
8. Crystallized N-terminal domain of influenza virus matrix protein M1 and method of determining and using same [R] . 2000

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Crystallization and prelimi - nary X-ray crystallographic analysis of yeast prion protein Ure2p with shortened N-terminal

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