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Cloning of lea cDNA fragment of carrot (Daucus carota L.) and analysis of its expression features

机译:胡萝卜lea cDNA片段的克隆及其表达特征分析

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摘要

Addition of concentrated sucrose to MS culture arrests the development of carrot somatic embryo at the stage of cotyledon embryo and, with the sucrose concentration restored to normal level, the embryo thus arrested is reactivated into post-embryonic development. Using the method of RT-PCR, the cDNA fragment of a new member of the Dc3 family of lea has been obtained from carrot somatic embryo under regulated state.
机译:在MS培养中添加浓蔗糖会在子叶胚阶段阻止胡萝卜体细胞胚的发育,并且在蔗糖浓度恢复到正常水平时,如此捕获的胚会重新激活为胚后发育。使用RT-PCR的方法,已从处于调控状态的胡萝卜体细胞胚中获得了Dc3家族的新成员lea的cDNA片段。

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