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Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression

机译:稻瘟病原菌诱导子嵌合诱导型启动子的构建及其表达

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摘要

The promoter fragments of wheat GstA1 and potato Gst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5' untranslated leader sequence together with GUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants.
机译:小麦GstA1和马铃薯Gst1的启动子片段已通过PCR扩增,分别克隆和融合至水稻肌动蛋白基因的最小启动子序列(Act1)及其5'非翻译前导序列以及GUS。为了分析其在转基因水稻植株中的诱导型,已将具有2个嵌合启动子(WGA和PGA)的构建体转移到水稻中。

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