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Expression of human erythropoietin directed by mWAP promoter in mammary gland of transgenic mice

机译:mWAP启动子指导的人类促红细胞生成素在转基因小鼠乳腺中的表达

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摘要

The present work has generated transgenic mice with a hybrid gene construct consisting of genomic sequences encoding human erythropoietin (hEPO) and governed by regulatory sequences of mouse whey acidic protein (mWAP). The construct proved effective by transient expression in lactating animal. After introducing hybrid gene construct into single-cell embryo via pronuclear microinjection, surviving embryo are reimplanted into pseudopregnant foster mother mouse.
机译:本工作已经产生了具有杂种基因构建体的转基因小鼠,该杂种基因构建体由编码人促红细胞生成素(hEPO)的基因组序列组成,并受小鼠乳清酸性蛋白(mWAP)的调控序列支配。通过在哺乳动物中的瞬时表达证明该构建体是有效的。通过前核显微注射将杂合基因构建体引入单细胞胚胎后,将存活的胚胎重新植入假孕寄养母鼠中。

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