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Agrobacterium-mediated transformation: state of the art and future prospect

机译:农杆菌介导的转化:最新技术和未来前景

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摘要

Great progress has been made in recent years in studies on the mechanism of Agrobacterium-mediated transformation and its application. Many details of the key molecular Events within the bacterial cells involved in T-DNA transfer have been elucidated, and it is notable That some plant factors which were elusive before are purified and characterized. Vast kinds of Species, which were either recalcitrant to or not included in the host range of Agrobacterium, can Now be transformed by this bacterium, and they include the very important cereal species, Gymnosperms, yeast and many filamentous fungi.
机译:近年来,在农杆菌介导的转化机理及其应用方面的研究取得了巨大进展。已经阐明了参与T-DNA转移的细菌细胞内关键分子事件的许多细节,并且值得注意的是,某些以前难以捉摸的植物因子已被纯化和鉴定。现在,这种细菌可以转化多种对农杆菌宿主有害或不参与农杆菌的物种,其中包括非常重要的谷物物种,裸子植物,酵母和许多丝状真菌。

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